蔬菜中有机磷农药残留的生物传感器法检测

Determination of Organo-phosphorus Pesticide Residues in Vegetables Using a Biosensor

将乙酰胆碱酯酶(AChE)固定在醋酸纤维膜上,将该膜组装在一支pH玻璃电极敏感膜表面,制成用于测定有机磷农药的电位型生物传感器,优化其制备方法。当溶液中存在有机磷农药,酶的活性受到抑制,酶活性的降低引起pH玻璃电极电位的改变,其改变程度与溶液中农药的浓度大小有关。用本文方法测定了蔬菜中的对硫磷、辛硫磷、氧乐果、敌敌畏,结果满意。

This paper refers to a potentiometric biosensor with a cellulose acetate membrane, on which acetylcholinesterase(AChE) is immobilized. The biosensor, based on the change of enzyme activity in the presence of pesticides, is applied to determination of organophosphorus pesticides such as parathion in vegetables.