脱细胞真皮基质结合表皮细胞悬液移植修复组织缺损

Use of allogenic acellular dermal matrix combined with autologous epidermal cells for the repair of tissue defect

目的探索一种修复组织缺损创面的方法。方法选46只成年Wistar大鼠为实验对象,实验组移植表皮细胞悬液,对照组用磷酸盐缓冲液代替移植物,将实验制备的异体脱细胞真皮基质移植到大鼠背部全层皮肤缺损创面,待其血管化后,在其上面移植自体表皮细胞悬液,定期观察表皮的形成,取标本行组织学检查,并行Ⅳ型胶原免疫组化染色以观察基底膜的重建。结果血管化的脱细胞真皮基质支持表皮细胞的增殖分化,能促进表皮-真皮连接的形成;表皮细胞移植后,在人为的体内培养环境中能够发育成完整的表皮;创面修复后的皮肤在组织学及外观上与脱毛后的正常皮肤近似,而且耐摩擦,有一定弹性,较接近正常皮肤。结论血管化异体脱细胞真皮基质结合自体表皮细胞悬液移植可用于修复组织缺损创面。

Objective To investigate a method for the repair of tissue defect. Methods Allogenic acellular dermal matrixes （ADM） were implanted to full-thickness skin defects made on the dorsa of rats. Two weeks later, autologous suspended epidermal cells were transplanted on to the surface of vascularized ADM. Respectively, neoepidermis was macroscopically observed 2, 3, 5 weeks after grafting, and samples were taken to make routine paraffin sections for microscopical examination, and immunohistochemical staining for type Ⅳ collagen was also performed. Results The vascularized ADM could support proliferation and differentiation of epidermal cells, and also could promote the formation of dermal-epidermal junction. Suspended epidermal cells in an artificial culture system in vivo could develop into mature epidermis. The reconstructed skin not only looked like the normal one in appearance in which hair was removed, but also revealed a better function. Conclusions Full-thickness skin defect can be repaired by transplanting autologous epidermal cell suspension on to vascularized ADM.