毛子草化学成分及其促PC-12细胞的分化作用研究Ⅰ

Study on chemical constituents from Incarvillea arguta and their accelerating PC-12 cell differentiation

目的:研究毛子草的化学成分及其促PC-12细胞的分化作用.方法:利用反复硅胶柱色谱进行分离和纯化,通过理化方法及光谱分析鉴定其结构.采用PC-12细胞株模型对毛子草不同提取部位及各单体化合物进行了促分化作用研究.结果:从毛子草的正丁醇溶解部分分离得到5个化合物,鉴定为:车前醚苷(Ⅰ),5-羟基-4',6,7-三甲氧基黄酮(Ⅱ),4',5-二羟基-6,7-二甲氧基黄酮(Ⅲ),4',5-二羟基-7-甲氧基黄酮(Ⅳ),5-羟基-4',7-二甲氧基黄酮(Ⅴ).结论:化合物Ⅰ为首次从该植物中分离得到;化合物Ⅱ～Ⅴ为首次从角蒿属植物中分离得到.生物活性表明毛子草正丁醇溶解部位和化合物Ⅰ对PC-12细胞均具有一定的营养活性.

Objective： To study chemical constituents of Incarvillea arguta and their accelerating PC-12 cell differentiation. Method：The constituents were isolated and repeatedly purified on silica gel column chromatography, and were identified on the basis of physicochemical and spectroscopic analysis. The neurotrophic activity of different portion and all purified compounds from Ⅰ. arguta was determined on the model of PC-12 cell. Result： Five compounds were isolated from BuOH portion of alcohol extraction of Ⅰ. arguta, Their structurs were identified as plantarenaloside （Ⅰ）, 5-hydroxy-4＇, 6 7-trimethoxy-flavone （Ⅱ）, 4＇, 5-dihydroxy-6, 7-dimethoxyflavone （Ⅲ）, 4＇,5-dihydroxy-7-methoxyflavone （Ⅳ）, 5-dydroxy-4＇, 7-dimethoxyflavone （Ⅴ）. Condusion： Compound Ⅰ is isolated from the plant for the first time and it has neurotrophic activity for PC-12 cell. Compounds Ⅱ-Ⅴ are isolated from the genus Incarvillea for the first time.