党参果聚糖的化学结构

Chemical structure of fructosan from Condonopsis pilosula

目的:对中药党参中多糖进行研究。方法:DE-52纤维素和Sephadex G-200凝胶柱色谱分离纯化一多糖CPP-1。凝胶色谱法测定其纯度和分子质量。酸全水解,甲基化反应,高碘酸盐氧化及降解,IR,1H-NMR和13C-NMR分析其结构。结果:CPP-1分子质量约为7.5×104,化学结构为呋喃果糖以-β(2→1)连接而成的果聚糖。结论:该多糖为一中性的均一多糖。

Objective： To study the polysaccharide of Condonopsis pilosula. Method： The polysaccharide, CPP-1, was purified by DE-52 cellulose and Sephadex G-200 gel column chromatography. Purity and molecular weight of the polysaccharide were determined by gel permeation chromatography. Methylafion analysis, periodate oxidation and degradation, IR, ^1H-NMR and ^13C-NMR methods were adopted to elucidate the chemical structure. Result： The molecular weight of CPP-1 was estimated to be 7.5×10^4, and the structure of CPP-1 was a β-（2→1）linked β-D-fructosan. Conclusion： CPP-1 was a neutral homosaccharide.