摘要
在常规临床实验室作尿草酸测定仍然有不少困难。本文报道一种测定尿草酸的比色法。草酸被锌还原成羟基乙酸,后者与变色酸反应显色,最大吸收波长570nm,草酸浓度在0~1000μg/L(终浓度)范围内与吸光度成线性,批内CV10.3%,批间CV17.3%,平均回收率94%,尿葡萄糖3g/L抗坏血酸100mg/L无干扰,正常参考值0.29~0.52mmol/24h尿,这些实验结果与国外文献报道一致。本法不需特殊设备,适合于一般常规临床实验室使用。
The measurment of oxalate in urine continues to be difficult.We described a colorimetric method of determing Urinary oxalate,Our obtained experiment data agree well with those reported in the literature. This is asuitable technigue for routine clinical laloratory.In the paper,we also introduced the clinical significance of hyperoxaluria.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1996年第1期5-7,共3页
Chinese Journal of Clinical Laboratory Science
关键词
草酸
尿结石
比色法
诊断
Oxalate,Urolith,Colorimetric method, Urine