人大肠癌雌激素受体的定量研究

Studies on the estrogen receptors in human coloretal carcinoma

应用葡聚糖包裹活性炭饱和分析法(DCC法)对30例原发性大肠癌手术切除标本行雌激素受体(ER)定量测定,同时取肿瘤组织(Tumor简称T)和其周围的正常粘膜(Normal Mucosa简称NM)分析其ER含量,结果发现T和NM中ER含量无显著性差异(P>0.05)。而且ER含量与性别、年龄、血型及肿瘤发生部位、组织类型、Dukes分期也未见有明显相关关系。结果提示,大肠癌组织中ER的存在是确实的,且癌周正常粘膜中也有较高的ER出现率。因此,人大肠癌可能为雌激素依赖性生长肿瘤,对ER阳性患者采用内分泌治疗可望有效。

The status of estrogen receptors (ER) in primary colorectal cancer was investigated by the dextran-coated charcoal (DCC) method. Thirty patients, operated on for primary carci- noma of large bowel, were studied. 12 were women and 18 were men, with mean age being 52 years (range: 14-85 years). They had never been treated by radiotherapy and chemothera- py. Tumors(T)and its surrounding noncancer- ous colorectal mucosa (NM) (>=10 cm apart from tumors tissue) were collected and mea- sured. In surrounding mucosa, estradiol recep- tors were detected in 80% of the patients (women 83%, men 78%, range 4.7%-90% fmol/mg protein). In tumors, ER were detect- ed in 73.3% of patients (range:0-77 fmol/mg protein). No difference was found between the ER of NM and T tissue (P>0.05), and ER content was not related to sex age, tumor site, histologic grade, stages of Duke's classification or blood type of patients. The results positive- ly confirmed the presence of ER in colorectal tumors and in the adjacent normal mucosa, suggesting that some large bowl cancer is en- docrine-dependent and the endocrine therapy on such patients may be effective.