应用PCR-SSCP银染技术检测结直肠癌P_53基因突变

Detection of p53 Gene Mutation in Colorectal Cancer With PCR-SSCP Sliver Staining Technique

应用PCR-SSCP银染技术检测30例结直肠癌P_53基因第5、6、7和8外显子的突变情况,结果发现16例(53.33%)发生P_53基因突变。提示:①结直肠癌普遍存在P_53基因突变。②比较传统的同位素标记PCR-SSCP技术,PCR-SSCP银染技术不仅灵敏度相当,而且简便、快速、低耗、重复性好,尤其适于大样本筛选,可在临床实验室中推广。

Using PCR-SSCP sliver staining tech- nique, we detected mutations in P53 gene ex- ons 5, 6, 7 and 8 in 30 patients with colorectal cancer. 16 out of 30 cases were P53 gene muta- tion positive which yielded a total mutation rate of 53.33% and exon 7 was found in 23.33% (7/30). Our results showed that P53 gene mutation was more common in colorectal cancer. Compared with isotopic PCR-SSCP, PCR-SSCP sliver staining technique is not on- ly equally sensitive but also simpler, and less expensive, which is especially useful in mass screening and in clinical laboratory.