检测三磷酸腺苷预测卵巢癌细胞株对化疗药物的敏感性

TheEvaluationofAdenosineTriphosphateBioluminescenceAssayforChemosensitivityTestingofOvarianCancerCelLine

目的：探讨三磷酸腺苷（ＡＴＰ）检测法作为卵巢癌体外药物敏感试验的可行性。方法：采用ＡＴＰ法，检测７种化疗药物对卵巢癌ＡＯ细胞株的抑制作用，并以四唑蓝显色反应（ＭＴＴ）法作为对照，分析ＡＴＰ法的稳定性、灵敏度及其测定时间。结果：（１）ＡＴＰ法与ＭＴＴ法相关性良好（ｒ＝０．９１８１），ＡＴＰ法测定的化疗药物对肿瘤细胞的抑制率，高于ＭＴＴ法测定的抑制率１０个百分点者占９０％，表明ＡＴＰ法的灵敏度高于ＭＴＴ法；（２）ＡＴＰ法结果稳定，方法简便，可定量；（３）以用药第五天进行ＡＴＰ法测定为宜。结论：ＡＴＰ法可作为一种新的化疗药物体外敏感试验方法。

Objective:Toassesstheeficacyofadenosinetriphosphate(ATP)assayforchemosensititytestingofovariancancercellineandtocompareitspredictingvaluewiththatofdiphenyltetrazoliumbromide(MTT)test.Methods:ByusingATPassayandMTTtestthecytotoxicefectof7anticancerdrugsonovariancancercellineAOweredetermined.ThesensitivityandstabilityofATPasaywerecomparedwiththoseofMTTtest.TheoptimaltimeofexposureofthecelstothedrugsbeforedoingATPchemosensitityasayisinvestigated.Results:(1)TheresultsobtainedbyATPasaycorelatewelwiththatbyMTTtest(r=0.9181).However,in90%oftheassaysamplesthecytotoxicefectofthechemotherapeuticagentsoncancercelsdetectedbyATPasaywas10%ormorestrongerascomparedwiththatdeterminedbyMTTtest.Thediferenceinsensitivityofthetwoassayswasalsodemonstratedbytheleastnumberoflivingcels.Achangeoflivingcelsof60perwelcouldbedetectedbyATPasaywhilethatdetectedbyMTTtestshouldbe200perwel.(2)Byrepeatingthetestsofasamebatchofsamplesfor5times,itrevealedthattheresultsobtainedbyATPassaywasmorestablethanMTTtest.Thediferencebetweenthesx(standarderor)ofthetwotestswassignificant(P<0.05).(3)Theoptimaltimeofexposureofthecancercelstotheanticancerdrugsfortestingitschemosensitivitywas5days.Conclusions:ThemethodofATPasayforchemosensitivitytestissimpleandconvenientwithhighsensitivityandstability.Itmaybeusedasanewinvitrochemosensitivitytestinpatientswithovariancancer.