摘要
本研究用 Sprague-Dawley 大鼠气管体外诱与鸡胚皮肤接触培养的方法,通过倒置显微镜、光镜和扫描电镜观察了蟾酥的抑癌作用。结果表明:(1)诱发性大鼠气管癌变细胞,具有向鸡胚皮肤侵袭性生长的生物学行为。同时证明蟾酥对大鼠气管体外诱癌过程具有抑制作用。(2)蟾酥具有逆转大鼠气管上皮的异型性鳞状化生,促进气管受损粘膜的修复和保护气管上皮正常分化的作用。(3)在 MCA 作用下可见纤毛细胞增生和化生。
The present investigation evaluated the inhibitory effects of Secretio Bufonis on the car- cinogenesis of rat trachea contacted with chick embryonic skin(CES)in culture under inverted microscopy,light microscopy and scanning electron microscopy.The experimental results showed that the malignant cells of tracheal rings revealed an invasion into the CES in the group M but the group N and group S did not.It suggested that Secretio Bufonis,might inhibit the carcinogenesis induced by MCA,reverse the atypical squamous metaplasia,promote the repair of tracheal mucosa and maintain normal differentiation of the rat tracheal epithelia.In addition,group M indicated the hyperplasia and metaplasia of some ciliated cells exposed to MCA. The mechanism of Secretio Bufonis against lung cancer arid the possibility of canceration of ciliated cells have been discussed.
基金
本课题由湖北省科委资助
