HPLC法测定小儿氨酚伪麻分散片的含量

Content Determination of Paracetamol and Pseudoephedrine Hydrochloride Dispersible Tablets for Children by HPLC

目的:采用高效液相色谱法测定小儿氨酚伪麻分散片的含量。方法:选用Kromasil C1815×0.46cm5μm色谱柱,甲醇-0.01mol·L^(-1)磷酸二氢钾-三乙胺(20∶80∶0.02),检测波长为215nm,流速为1.0ml·min^(-1)。结果:对乙酰氨基酚在1.039~4.155μg范围内呈线性关系;盐酸伪麻黄碱在0.0968~0.3873μg范围内呈线性关系。结论:该法具有准确,简便,快速,灵敏等特点。

Objective：A high performance liquid chromatography method was established to determine the content of Paracetamol and Pseudoephedrine Hydrochloride Dispersible Tablets for Children, Methods：The determination was carried out with Kromasil C18 （ 15 × 0.46cm, 5μm ） column, using methanol-potassium dihydrogen phosphate （ 0.01mol · L^-1 ）-triethylamine （20 ： 80 ： 0.02）as the mobile phase. The flow rate was controlled at 1ml · min^-1 and detected wavelength at 215nm. Result：The linear range of Paracetamol was from 1. 039 to 4. 155μg and the linear range of Pseudoephedrine Hydrochloride was from 0. 09683 to 0. 3873μg. Conclusions：This HPLC method was precise,efficient ,fast,as well as sensitive.