摘要
目的:建立女宝胶囊中延胡索乙素的含量的检测方法.方法:采用柱C18(4.6×250mm,5μm),甲醇-0.1%磷酸溶液(用三乙胺调pH至6.0)(60:40)为流动相,流速:1.0ml/min,检测波长:280nm.结果:延胡索乙素的量在0.102～1.632μg范围内线性良好,回收率为98.78%,RSD为2.75%.结论:该方法准确,可靠,可用于女宝胶囊的质量控制.
Objective：To establish a method of HPLC assay for determining tetrahydropalmatine in Nübao Capsule. Metbod： A Hypersil ODS column（4.6×250μm）was used with a mobile phase of a mixture of methanol-0.1% phosphate acid （adjust pH to 6.0 with triethylamine）（60 ： 40）. The flow rate was 1.0ml/min. A detection wavelength at 246nm was used. Result： The calibration curve of tetrahydropalmatine was linear between 0. 102-1. 632μg. The mean recovery was 98. 78% with RSD 2.75%. Conclusion ：This method was proved to be simple and reliable,and can be used for the quality control of Nübao Capsale.
出处
《中国药品标准》
CAS
2005年第4期24-25,41,共3页
Drug Standards of China
