寡聚酰胺PyPyPyβDp与DNA相互作用的荧光动力学被引量：1

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摘要通过稳态吸收光谱、荧光光谱和时间分辨荧光光谱对寡聚酰胺分子PyPyPyβDp(简称PPP)的光物理性质进行了详细研究.发现PPP的激发态性质受溶剂环境影响较大,在TKMC缓冲液中,PPP有较弱的荧光,其荧光寿命为16ps;随着溶剂极性逐渐降低,荧光光谱蓝移,荧光强度增加,相应荧光寿命增长.通过对PPP与DNA双螺旋分子相互作用的荧光动力学研究表明,PPP与DNA相互作用后,荧光强度增强,荧光寿命从16ps增加到32ps,证实了存在PPP与双螺旋DNA的结合相互作用.

作者张慧娟王津吴义室袁谷艾希成汪力张建平

机构地区中国科学院化学研究所分子动态与稳态结构国家重点实验室北京大学化学与分子工程学院中国科学院物理研究所凝聚态物理中心

出处《中国科学（B辑）》 CSCD 北大核心 2005年第4期275-279,共5页 Science in China(Series B)

基金国家自然科学基金资助项目(批准号:20133020 20272005)

关键词寡聚酰胺(PPP) 小牛胸腺DNA(ctDNA) 时间分辨荧光光谱识别动力学分子相互作用 DNA双螺旋荧光动力学寡聚酰胺 DP 光物理性质

分类号 Q523 [生物学—生物化学]

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参考文献16

1Dervan P B. Molecular recognition of DNA by small molecules.Bioorgamic & Medicinal Chemistry, 2001, 9:2215-2235.
2Rucker V C, Foister S, Melander C, Dervan P B. Sequence specific fluorescence detection of double strand. J Am Chem Soc,2003, 125(5): 1195-1202.
3Zimmer C, Wahnert U. Nonintercalating DNA-binding ligands:Specificity of the interaction and their use as tools in biophysical,biochemical and biological investigation of the genetic material.Prog Biophys Mol Biol, 1986, 47:31-112.
4Chiang S Y, Welch J, Rauscher F J, Beerman T A. Effects of minor groove binding drugs on the interaction of TATA box binding protein and TF II A with DNA. Biochemistry, 1994, 33:7033-7040.
5Kopka M L, Yoon C, Goodsell D, Pjura E Dickerson R E. The molecular origin of DNA-drug specificity in netropsin and distamycin. PNAS, 1985, 82:1376-1380.
6Pelton J K, Wemmer D E. Structural characterization of a 2:1 distamycin A/d(CGCAAATTGGC) complex by two-dimensional NMR. PNAS, 1989, 86:5723-5727.
7Lown J W, Krowicki K, Bhat U G, Skorobogaty A, Ward B, Dabrowiak J C. Molecular recognition between oligopeptides and nucleic acids: novel imidazole-containing oligopeptides related to netropsin that exhibit altered DNA sequence specificity. Biochemistry. 1986. 25:7408-7416.
8Mrksich M, Wade M S, Dwyer T J, Geierstanger B H, Wemmer D E, Dervan P B. Antiparallel side-by-side dimeric motif for sequence-specific recognition in the minor groove of DNA by the designed peptide 1-methylimidazole-2 carboxamide netropsin.PNAS, 1992, 89:7586-7590.
9Wade M S, Mrksich M, Dervan P B. Binding affinities of synthetic peptides, pyridine-2-carboxamidonetropsin and 1-methy-limidazole-2-carboxamidonetropsin, that form 2:1 complexes in the minor groove of double-helical DNA. Biochemistry, 1993, 32:11385-11389.
10Dervan P B, Burli R W. Sequence-specific DNA recognition by polyamides. Curr Opin Chem Biol, 1999, 3:688-693.

二级参考文献13

1Holmlin R E, Dandliker P J, Barton J K. Charge transfer through the DNA base stack. Angew Chem Int Ed Engl, 1997, 36:2714-2730
2Kelley S O, Barton J K. Electron transfer between bases in double helical DNA. Science, 1999, 283(15): 375-381
3Wan C, Fiebig T, Schiemann O, et al. Femtosecond direct observation of charge transfer between bases in DNA. PNAS, 2000,97(26): 14052-14055
4Giese B, Amaudrut J, Kohler A K, et al. Direct observation of hole transfer through DNA by hopping between adenine bases and by tunneling. Nature, 2001,412(19): 318-320
5Giese B. Long-distance charge transport in DNA: The hopping mechanism. Acc Chem Res, 2000, 33(9): 631-636
6Guest C R, Hochstrasser R A, Sowers L C, et al. Dynamics of mismatched base pairs in DNA. Biochemistry, 1991, 30(13):3271-3279
7Saito I, Nakamura T, Nakatani K, et al. Mapping of the hot spots fir DNA damage by one-electron oxidation: Efficacy of GG doublets and GGG triplets as a trap in long-range hole migration. J Am Chem Soc, 1998, 120(48): 12686～12687
8Seidel C A M, Schulz A, Sauer M H M. Nucleobase-specific quenching of fluorescence dyes. 1. Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies. J Phys Chem, 1996, 100(13): 5541～5553
9Fiebig T, Wan C, Zewail A H. Femtosecond charge transfer dynamics of a modified DNA base: 2-aminopurine in complexes with nucleotides. Chem Phys Chem, 2002, 3:781-788
10Hodes R. Molecular targeting of cancer: Telomeres as targets.PNAS, 2001, 98(14): 7649-7651