用NMR技术研究蛋白质-配体相互作用

Mapping Protein-Ligand Interaction by NMR Techniques: A Review

蛋白质配体相互作用的研究对理解生命过程、药物设计和药物筛选具有相当重要的科学意义和巨大的经济价值.NMR是研究蛋白质配体相互作用的最有用的技术之一,有着显著的优势.本文综述了近年来国际上用NMR技术研究蛋白质配体相互作用的发展状况和趋势,先介绍表征蛋白质配体相互作用的重要参数,然后介绍如何判断蛋白质或配体与复合物的化学交换类型以及所能获得的有关蛋白质配体相互作用的信息,最后介绍具体用于研究蛋白质配体相互作用的若干NMR技术以及基于NMR的药物筛选技术.

Protein-ligand interaction studies play an important role in understanding of biological science, drug screening and drug design, thus have both scientific and economic meanings. In this review, NMR techniques that are being widely employed to study protein-ligand interaction are summarized. The important parameters used to characterize the intermolecular interaction are introduced firstly, followed by descriptions of the approaches for identifying interaction regions and how to extract valuable information related to intermolecular interaction. Finally, the NMR techniques that have been applied to map protein-ligand interaction are introduced with application examples.