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Ⅰ型马立克氏病病毒Md11株pp24基因的真核表达

Eukaryotic expression of pp24 gene of Md11 strain of marek's disease virus
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摘要 通过PCR方法扩增MDV Md11株的pp24基因,将其克隆到真核表达载体pcDNA3.1/zeo(+)中。阳性克隆鉴定后,在脂质体作用下转染CEF细胞,用间接免疫荧光试验检测,结果证明阳性克隆在CEF细胞中表达了pp24蛋白。 pp24 gene of Marek＇s Disease Virus（MDV） Mdll strain was amplified by polymerase chain reaction and cloned into pcDNA3.1/zeo（ ＋ ）.The recombinant plasmid was transfected into chick embryo fibroblasts（CEF）. Indirect immunofluorescence assay （IFA）showed that pp24 gene expressed the 24 kD phosphorylated protein which can response to Mouse-Anti-GST-pp24 sera.
作者 姜世金 杨汉春 崔治中
机构地区 山东农业大学动物科学院 中国农业大学动物医学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2005年第5期326-328,共3页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金(30070544)
关键词 MDV pp24 转染 IFA MDV pp24： transfection IFA
分类号 S852.659.4 [农业科学—基础兽医学]
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参考文献10

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二级参考文献26

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中国预防兽医学报
2005年 第5期

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