猪母乳抗E2抗体水平的调查研究

Research into the level of anti-E2 antibody in female milk

本实验对采至广西某猪场的270份母乳奶样,应用间接ELISA和间接血凝试验,分别对其进行了抗猪瘟E2抗体水平和猪瘟全抗体水平的检测。检测结果显示,在270份样品中,间接ELISA检测呈阳性的(即含有抗E2抗体的)占总样品的94.45%,而间接血凝试验检测出这些样品的效价在1∶16以上(具有保护力的)高达97.78%。从实验结果可以看出,该猪场母猪在哺育阶段的母乳含有抗E2抗体,仔猪能从母乳获得抗体,从而获得对猪瘟的免疫力。从两个试验的检测结果对照可知,两个数据差异不显著,说明用间接ELISA检测抗E2抗体、用间接血凝试验检测猪瘟抗体来监测猪瘟抗体免疫水平是可行的。本实验进一步论证了E2抗体就是猪瘟的保护性抗体,同时为监测猪瘟开创了一种新的可行方法,这对监测猪瘟真正的免疫水平,帮助猪场制定有效的免疫程序具有很现实的重要的意义。

This experiment with 270 milk samples that adopt to the some pig field in Guangxi,apply indirect ELISA and indirect hemagglufinafion test. Carry on exam the anti-E2 antibody level and the swine fever antibody level. The examination result shows that 270 samples in the indirect ELISA test reports the positive （exist the anti-E2 antibody） accounting to 94.45 % of total samples while the indirect hemagglutination test shows that the potency above 1：16 accounting for 97.78 % .We can know from the experirnent result,female milk exist anti-E2 antibody during feed stage in this pig field, the piggy can acquire the antibody from the milk, them acquire the immunity ability to resist the swine fever. Check against from two result the difference between the two data is not obvious. It is proved that the indirect ELISA measures the anti-E2 antibody and use the indirect hemagglutination test swine fever, monitor the swine fever antibody level is viable. Further argument in this experiment the antibody of E2 is the protection antibody of the swine fever, at the same time for monitor swine fever,found a new valid method, to monitors the swine fever true immune lever. It is significant to help the pig field establish valid immunity procedure etc.