中国健康汉族和瑶族人群巯基嘌呤甲基转移酶活性的分布

Distribution of thiopurine methyltransferase activity in Chinese healthy Han and Yao population

目的研究巯基嘌呤甲基转移酶(TPMT)活性在中国汉族和瑶族人群中的分布。方法建立改良的反相高效液相色谱法,测定人红细胞(RBC)中TPMT活性。结果测定了273名中国健康汉族成年人、88名汉族儿童和148名健康瑶族儿童红细胞中TPMT活性,未发现酶缺陷者,其平均活性分别为(11.96±3.27),(13.02±2.78)和(13.27±3.92)nmol·(h·mL)-1packed红细胞。瑶族儿童TPMT活性较同龄汉族儿童差异无显著性;TPMT活性在中国汉族和瑶族人群中都呈正态分布;在中国汉族和瑶族人群中均未发现有与性别有关的差异;汉族人群TPMT活性与年龄无关;瑶族儿童TPMT活性与年龄呈正相关,但相关关系较弱。结论中国汉族和瑶族人TPMT活性分布均呈正态分布。

Objective To investigate thiopurine methyhransferase （TPMT） activity distribution in Chinese Han and Yao population. Methods TPMT activity of erythrocyte （RBC） in healthy subjects was measured by RP - HPLC method. Results The mean values of TPMT activity were （ 11.96 ± 3.27） nmol · h^-1 . mL^-1 packed RBC in 273 healthy Chinese Han adults, （ 13.02 ± 2.78） nmol · h^-1 . mL^-1 packed RBC in 88 healthy Chinese Han children, and（13.27 ± 3.92）nmol · h^-1 · mL^-1 packed RBC in 148 healthy Chinese Yao children, respectively. No subject had hardly detectable erythrocye TPMT activity. The frequency distribution histogram in Chinese Han and Chinese Yao children appeared to be normal. There was no statistically significant in TPMT acitvity between Chinese Yao children and Han children, and there was no gender differences in TPMT activity. There was no significant agerelated differences in TPMT activity in the Chinese Han adults and Chinese Han children. However, TPMT activity correlated positively with age in Chinese Yao children with a small value of γ （γ= 0. 2596）. Conclusion The frequency distribtion of TPMT activity was unimodal in Chinese Han and Yao population.