用灿烂甲酚蓝褪色分光光度法测定肝素钠的研究

Fading Spectrophotometric Determination of Heparin with Brilliant Cresyl Blue

在pH 3.0的Britton-Robinson (B-R)缓冲溶液中, 用分光光度法研究了灿烂甲酚蓝与肝素钠的相互作用. 灿烂甲酚蓝在592 nm处有一特征吸收峰, 加入肝素钠后, 吸收峰强度降低但没有新的吸收峰出现, 表明两者能够相互结合形成复合物. 在最佳条件下, 利用溶液吸光度值的降低与肝素钠浓度的关系建立了测定肝素钠浓度的分光光度法, 线性范围为0.6～6.0 mg·L-1, 摩尔吸光系数ε=1.03×106 L·mol-1·cm-1, 检测限(3σ)为0.173 mg·L-1. 将该方法应用于肝素钠注射液浓度的测定, 结果令人满意.

A new fading spectrophotometric method for determining trace heparin is proposed in the present paper. In pH 3.0 BrittonRobinson （B-R） buffer solution, brilliant cresyl blue can react with heparin resulting in the decrease of absorbance of BCB at 592 nm. The conditions for the interaction were optimized, and the interferences of coexisting substances were investigated, Under the optimal conditions the reduction of the absorbance is proportional to the concentration of heparin in the range of 0.6-6.0 mg· L^-1 with the detection limit of 0.173 mg·L^-1. The molar absorptivity of the method was calculated to be 1.03 × 10^6 L· mol^-1 cm^- 1. The method has been applied to determine the heparin sodium injection solution with satisfactory results.