大鼠严重胸部创伤肺泡与间质巨噬细胞分泌TNFα-、IL-6的差异及意义

Effect of severe thoracic trauma combined with endotoxin infection on secretory function of alveolar macrophages and pulmonary interstitial macrophages in rats

目的观察大鼠严重胸部创伤后肺泡(AM)及间质(IM)巨噬细胞两类亚群分泌TNF-α、IL-6的差异,并探讨其意义。方法利用小型多功能生物撞击机,以400kPa驱动压力对大鼠右侧上胸壁进行致伤,建立大鼠严重胸部创伤模型,支气管肺泡灌洗,机械结合酶消化法分离、培养肺泡及间质巨噬细胞,动态检测创伤前、创伤后2、4、8、16、24小时以及复合LPS攻击后肺泡及间质巨噬细胞分泌TNF-α、IL-6的水平。结果创伤复合LPS攻击后,AM、IM分泌TNF-α、IL-6增加,伤后4、8、16小时时相点AM分泌TNF-α显著高于IM;伤后每个时相点IM分泌IL-6均显著高于AM。结论大鼠严重胸部创伤对AM、IM分泌TNF-α、IL-6具有不同影响,其在创伤后机体免疫功能紊乱的过程中具有不同作用,本研究为创伤性急性肺损伤的发病机制提供了一定的实验及理论依据。

Objective To observe the different changes of alveolar maerophages and interstitial macrophages on secretory function. Methods Rat models of severe thoracic trauma were established,alveolar macrophages were collected from bronehoalveolar lavage fluid, mechanic flush and enzymatic digestion, and then separated and cultured. Secretory funetion of AM and IM were observed dynamically before trauma and 2,4,8,16,24h after trauma and ehallenged by LPS. Results A stable and reliable severe thoracic trauma model was successfully established with 400kPa-strike on the up-right ehest of rat by a multiple-function strike apparatus. Severe thoraeie trauma and LPS ehallenged increased TNF-α、 IL-6 secretion both in AM and IM. AM secreted more TNF-α than IM at 4,8,16h after trauma,and IM secreted more IL-6 than AM at every time point after trauma. Conclusion There effect of severe thoracic trauma on seeretion of TNF-α.IL-6 between AM and IM is different. The present study provides valuable laboratorial and theoretieal evidence for the research on ALI complieated with severe thoraeic trauma.