混合胸腺移植防止器官特异性自身免疫损害的实验研究

Prevention of organ specific auto-immune disease by mixed fetal syngeneic or allogeneic and xenogeneic thymus

目的探讨同系或同种胸腺与异种胸腺混合移植防止器官特异性自身免疫损害的可行性。方法BALB/c裸小鼠的肾包膜下植入胸腺建立异种、同系与异种混合、同种与异种混合胸腺移植模型。术后6个月内比较各组发生自身免疫性疾病情况,观察胃的组织学变化,并用ELISA检测受体血清抗DNA自身抗体及用间接免疫荧光法检测的自身抗体。结果异种组80%发生自身免疫性疾病而死亡,血清中自身抗体明显高于混合组,异种组胃组织有明显的炎性损害而混合组未观察到明显的炎性改变。结论异种胸腺植入裸小鼠可引起宿主自身免疫损害,而同系或同种异种混合胸腺移植能够有效阻止自身损害的发生。

Objective To explore feasibility of prevention of organ specific autoimmune disease by transplanting mixed fetal syngenele or allogeneic and xenogeneic thymus. Methods The xenogeneie thymus grafting, the mixed syngeneic and xenogeneic thymus grafting, the mixed allogeneic and xenogeneic thymus grafting models in nude mice were established. Incidence of auto-immune disease between the xenogeneic group and the mixed thymus group was compared. Anti-DNA auto-antibodies and anti-gastric auto-antibodies of recipient＇s sera were tested by ELISA and indirect immunofluoreseence assay with gastric frozen sections from normal adult BALB/c mice,and histological changes of recipient＇s organs and tissues were observed simultaneously. Results Eighty percent of recipients died of auto-immune disease. Inflammatory lesions in the stomachs in xenogeneic thymus transplantation group were observed hut not found in the mixed group. Level of anti-DNA auto-antibodies and anti-gastric autoantibodies in the sera of xenogeneic group recipients were significantly higher than that of the mixed group. Conclusion Mixed syngeneic or allogeneic and xenogeneie thymus grafting can prevent the autoimmune lesions.