急性白血病患者Bcl-2/Jh基因的研究

Detection of Bcl-2/IgH Fusion Gene of Acute Leukemia Patients

目的探讨急性白血病(AL)患者t(14;18)融合基因Bcl-2/Jh突变点,在AL诊治过程中的变化及预后判断的意义.方法采集AL患者和正常人外周血,PCR方法检测Bcl-2/Jh基因3种不同重排方式的发生率.结果完全缓解期白血病患者体内mbr、mcr和mdr的发生率分别为46.6%、41.2%和13.8%.发生3种易位的机率为1.72%、2种易位的机率为5.2%.结论Bcl-2/Jh在AL患者治疗前后的发生率和重排率虽有不同,可在一定程度上反映疗效.

Objective To detect the Bcl - 2/Jh fusion gene caused by t（ 14 ; 18） translocation in acute Jeukemia patients and evaluate the clinical implications of bel - 2/Jh gene rearrangement in the diagnosis and therapy of leukemia. Methods Three types of Bcl - 2/Jh gene rearrangement analysis in fresh peripheral blood samples was padormed in patients with leukemia or healthy people by polymerase chain reaction（PCR）. Results The appearance of bcl - 2/Jh gene rearrangement rate was mbr 46.6% ,mcr41.2%and 13,8% in patients with leukemia in remission The sumultaneity appearance of three types gene rearrangement was 1.72% respectively, the sumultaneity appearance of two was 5.2%. Conduslons Although the be｜ - 2/Jh gene rearrangement has different rate fore - and aft chemotherapy in acute leukemia,it may reflect the effect to some extend ,further large sample quantitive analysis should be conducted to be used in clinical diagnosis and monitoring prognosis.