急性白血病骨髓基质细胞生物学特点的观察

Observation on the biological behavior of acute leukemia bone marrow stroma cells

目的探讨白血病骨髓基质对白血病形成的作用机制。方法白血病患者和正常人的骨髓单个核细胞行基质细胞培养,动态观察贴壁时间、小丛形成时间、集落形成时间、融合形成时间等生长特性,计数CFU-F集落,检测基质细胞VCAM-1、Fn及培养上清液中的IL-6、TNF-α含量。结果急性白血病骨髓基质细胞生长延迟,贴壁形成时间、小丛形成时间、集落形成时间和融合形成时间均明显晚于正常骨髓基质组(P<0.05);CFU-F计数和VCAM-1表达水平均明显低于正常对照组(P<0.05),Fn与正常对照组无显著性差异(P>0.05);骨髓基质细胞培养上清液中IL-6、TNF-α含量明显高于正常对照组(P<0.05)。结论急性白血病存在造血微环境异常。

Objective To study the mechanism of acute leukemic bone marrow stroma in leukemia development. Methods With the method of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cells, investigate the growth properties of leukemic bone marrow stroma, the expression of VCAM-1 （vascular cell adhesion molecule-1 ）, Fn（fibronectin） and the content of IL-6（interleukin-6） ,TNF-α（tumor necrosis factor-alpha） in culture medium. Results The growth of acute leukemic bone marrow stromal cells was delayed. The time of sticking to wall, the time of microplexus formation, the time of colony formation, the time of confluence formation, were all significantly delayed （P〈 0. 05）. The number of CFU-F and the content of VCAM-1 in stromal cells was respectively significantly decreased with that of controls（P〈0.05）,in addition, the content of Fn in stromal cells from acute leukemia was identical with that of controls（P〉0.05 ）, whereas, the content of IL-6 and TNF-α in the culture medium from acute leukemic bone marrow stromal cells was significantly increased compared with that of controls. Conclusion There were many abnormalities in hematopoietic inductive microenvironment（HIM）of acute leukemia.