小鼠TPO cDNA转染前后骨髓基质细胞TPOmRNA表达情况的研究

Experimental study on the expression of TPOmRNA in bone marrow stromal cells of mice before and after transfection

目的研究小鼠促血小板生成素(TPO)cDNA转染骨髓基质细胞(BMSCs)前后BMSCs中TPOmRNA表达的情况。方法以脂质体介导法将TPOcDNA真核表达质粒转染至BMSCs,用RT-PCR方法检测TPOmRNA的表达。结果转染TPOcDNA的BMSCs其TPOmRNA表达量明显高于空载体组(P<0.01)及未转染组(P<0.01)。结论阳离子脂质体能有效介导TPOcDNA真核表达质粒转染BMSCs,且转染后BMSCs中TPOmRNA的表达显著上调。

Objective To investigate the expression of TPOmRNA in TPOcDNA-modified bone marrow stromal cells（BMSCs） of mice before and after transfection. Methods With transfection of TPOcDNA eukaryotic expressing vector encapsulated lipofectamineTM 2000 into BMSCs. Using the method of RT-PCR to detect the expression of TPOmRNA. Results The expression of TPOmRNA in TPOcDNA-modified BMSCs was significantly enhanced compared to no-TPOcDNA plasmid and no-transfected BMSCs （P〈0.01）. Conclusion TPOcDNA eukaryotic expressing vector could be transfected into BMSCs efficiently by lipofectamineTM 2000 and the expression of TPOmRNA upregulated in TPOcDNA-modified BMSCs.