基因工程灭蚊幼蓝藻的现场初步观察

Preliminary Field Observation on Genetically Engineered Larvicidal Cyanobacterium

将转入球形芽孢杆菌毒蛋白基因表达质粒的工程藻Ａｎａｂａｅｎａｓｕｂｔｒｏｐｉｃ（ｐＤＣ２６）大量培养后，应用于山东省平阴县进行现场观察。结果表明：工程藻在９．４１×１０５ｃｅｌｌｓ／ｍｌ浓度以上时，４８ｈ可杀死９９～１００％蚊幼虫；在９．１３×１０４ｃｅｌｌｓ／ｍｌ浓度时，４ｄ可杀死９９％以上蚊幼虫，连续观察２个多月，工程藻仍有明显杀蚊效果，实验组最多１４条／勺幼虫，而对照组２１０条／勺左右。观察还发现，工程藻对库蚊幼虫杀灭效果最好，其次为伊蚊，但高浓度时，对伊蚊仍有明显杀灭效果；由于按蚊幼虫密度太低，未能观察。此结果比单独使用球形芽孢杆菌杀灭蚊幼虫有明显提高，持续时间也延长，这将为蚊虫的持续控制提供一个新的有力武器。

After Anabaena subtropic (pDC26) of engineered larvicidal cyanobacterium was cultured in the large,its larvicidal effect was observed in the fileds of Pingyin county of Shandong Province,The results showed that A.subtropic(pDC26) had high toxicity against the larvae of Culex pipiens and Aedes albopictus,it killed 99 100% larvae in 48hrs at the concentration of 9.41×105,and 8.95×106,and above 99% in 72h at 9.13×104. It had obviously toxicity against the mosquito larvae continuously for two months,The effect of toxicity against the larvae of Culex pipiens was better than that of Aedes albopictus.Therefore the genetically engineered cyanobacteria opened up new possibilities for mosquito control.