摘要
This research used confocal laser scanning mi-croscopy to examine the initiation and connection of vessel elements in Arabidopsis thaliana (L.) Heynh seedlings. The results indicated that vessel elements differentiated first in the lower portion of cotyledon-node zone (CNZ) 2 h after germination, and then extended downwards to the hypocotyl and root and upwardly to the middle portion of CNZ. 10 h after germination, vessel elements developed in the lower portion of cotyledon blade, the second initiating site, con-necting gradually with that initiated in cotyledon node and forming pinnate-marginal venation. The connection of vessel elements occurred between the upper portions of CNZ and epicotyl-shoot zone 7 d after germination. By then, the com-plete connection of vessel elements in the axial and lateral organs had formed.
This research used confocal laser scanning microscopy to examine the initiation and connection of vessel elements in Arabidopsis thaliana (L.) Heynh seedlings. The results indicated that vessel elements differentiated first in the lower portion of cotyledon-node zone (CNZ) 2 h after germination, and then extended downwards to the hypoeotyl and root and upwardly to the middle portion of CNZ. 10 h after germination, vessel elements developed in the lower portion of cotyledon blade, the second initiating site, connecting gradually with that initiated in cotyledon node and forming pinnate-marginal venation. The connection of vessel elements occurred between the upper portions of CNZ and epieotyl-shoot zone 7 d after germination. By then, the complete connection of vessel elements in the axial and lateral organs had formed.
基金
This Tesearch was supported by National Natural Science Foundation of China(Grant No.30370084)
Doctorate Vesting Point Foundation of the Education Committee of China(Grant No.20020019034)
Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences.
关键词
树苗
导管要素
激光扫描
叶子
萌芽过程
Arabidopsis, seedling, vessel element, initiation, connection.