赤芍801对大鼠心脏和肝脏微粒体羧基酯酶的诱导作用

Effect of Radix Paeoniae Rubra 801 on Induction of Cardiac and Hepatic Microsomal Carboxylesterases Activities in Male Rats

心脏和肝脏微粒体羧基酯酶(CEase)的活力用醋酸硝基苯水解后释放的对硝基苯酚的量表示。本研究观察了酶诱导剂苯巴比妥(PB)、地塞米松(DEX),安妥明(CPIB)以及赤芍801、首乌、茵陈对大白鼠心脏和肝脏 CEase 活力的诱导作用。结果表明 PB、DEX、CPIB、赤芍801、茵陈对肝脏 CEase 活力均有诱导作用(P 均<0.05),而 PB、DEX、CPIB 使肝脏重量增加(P 均<0.05),赤芍801、茵陈对肝重量没有影响。DEX、CPIB、赤芍801对心脏 CEase 活力有诱导作用(P 均<0.05)。研究提示赤芍801、茵陈对肝脏水解过程有促进作用,有利于毒物排泄。

Cardiac and hepatic microsomal carboxylesterases(EC.3.1.1.1,CEase)activities were showed by measuring the released P-nitrophenol resulting from hydrolysis of P-nitrbphenylacetate.Induc- tion effects of inducers,such as PB,DEX,CPIB and Radix Paeoniae Rubra 801(RPR 801),Radix Polygoni Multiflori(RPM),Herba Artemisiae Scopariae(HAS)on cardiac and hepatic CEase activities were observed.In the study,RPR 801 and HAS just like PB,DEX,CPIB had the induction effect on hepatic CEase activity(P<0.05);the liver weight could be increased by PB,DEX and CPIB (P<0.05)and could not be affected by RPR 801 and HAS;RPR 801 just like DEX,CPIB had the induction effect on cardiac CEase activity(P<0.05).The results suggested that RPR 801 and HAS could promote the hydrolysis process in liver and help to excrete of toxic substance.