青霉素发酵罐的接种工艺改进

Improved inoculation of penicillion fermenter

目的改进青霉素发酵罐的接种工艺,提高青霉素生产水平。方法采用不同接种量、不同接种方式的对照实验和正交化实验,改进接种工艺和与之匹配的发酵前期工艺。结果采用两种种源混合接入,总接种量为30%,其中种罐种子液的接种量为20%,发酵至60h的前期发酵液的接种量为10%。结论接种工艺改进后,发酵指数提高10.8%,提炼收率提高0.4%,发酵提炼的生产指数提高11.2%。

Objective To improve inoculation of penicillion fermenter and to increase level of penicillion production. Methods Inoculation and accordant earlier fermentation process were optimized by comparison and orthogonal design experiments. Results It was adopted that overall inoculation volume of two types seeds was 30%, respectively, the inoculation of seed broth was 20% and the one of fermentation broth of 60th hour was 10%. Conclusions It was showed that fermentation index was increased by 10.8%, refinement yield 0.4%, and production index of fermentation and refinement 11.2%.