长春丹对老年大鼠脑和血浆中去甲肾上腺素水平的影响

Effect of Chang Chun Dan()on Noradrenaline Level of Brain and Plasma in Aged Rats

用放射酶法测定青年组、老年组和服药老年组大鼠下丘脑和血浆去甲肾上腺素(NA)水平的变化;用组织化学方法观察蓝斑核内 NA 荧光强度的变化。结果发现长春丹可使老年大鼠下丘脑 NA 含量下降,血浆 NA 含量有上升趋势;而对蓝斑核内 NA 的荧光强度无明显作用。从上述中枢与外周 NA 的变化分析了补肾益气药长春丹对老年机体交感神经系统和内分泌功能的作用。

In this paper,the rats were randomly divided into three groups:(1)Young(Y);(2)Aged(A);(3) Aged rats treated with Chang Chun Dan(AT)for 3 months.The level of noradrenaline(NA)in brain and plasma of three group rats had been examined by radioenzymatic assay.The changes of fluorescent intensity of NA in locus coeruleus had been observed by histochemical method.The levels of NA in hypothalamus of three groups were 162±9.93,209±13.2,167±11.8 ng/g wet tissues and in plasma were 1.29±0.16,0.84±0.08,0.96±0.08 ng/ml respectively.The results showed that the NA level of hypothalamus in AT group was lower than that of A group(P<0.05),and the NA level in Y group was raised markedly comparing with A group(P<0.01).The plasma NA Ievel in A group Was significantly lower than that in Y group(P<0.05).The NA level in AT group was raised a little comparing with A group.But the fluorescent intensity of NA in locus coeruleus between AT and A group was no difference.The results suggested that the changes of neurotransmitter induced by Chang Chun Dan may be basis of delaying aging effect and improving symptom of Kidney deficiency induced by aging.