IL-10对大鼠脊髓损伤后内皮细胞ICAM-1表达作用

EFFECT OF IL-10 ON ICAM-1 FOLLOWING SPINAL CORD INJURY IN MICE

①目的探讨IL-10对继发性脊髓损伤(SCI)后脊髓血管内皮细胞表面表达的细胞间黏附分子-1(ICAM-1)的作用.②方法建立大鼠SCI模型,用免疫组织化学方法观察IL-10及甲基泼尼松龙(MP)对大鼠SCI后1、4、12、24、48、72、120 h脊髓内皮细胞ICAM-1表达的影响.③结果大鼠SCI后1 h未见ICAM-1的表达,4 h脊髓损伤部位的内皮细胞开始出现ICAM-1的表达,24～48 h达高峰,然后逐渐下降,120 h仍可见到ICAM-1的表达.IL-10和MP对脊髓损伤部位的内皮细胞表达的ICAM-1都有明显地抑制作用,两者合用则更加明显地抑制了ICAM-1的表达.④结论 ICAM-1参与了SCI损伤早期的病理过程,是引起SCI继发性损伤病因中的重要因素之一.SCI后早期应用IL-10及MP阻断ICAM-1的表达有助于减轻SCI的损害程度.

Objective To evaluate the effect of IL-10 on intercellular adhesion molecular-1 （ICAM-1） expressed on the surface of spinal cord blood vessels endothelium cells after secondary spinal cord injury. Methods Immunohistochemistry was used to observe the expression changes of ICAM-1 as a result of IL-10 and methyllprenisonlone （MP） 1,4,12,24,48,72, and 120 hours after SCI, respectively. Results ICAM-1 expression was not observed one hour after SCI, which began to appear in the site of injury four hours later, reached its peak after 24-48 hours, and then decreased gradually. ICAM-1 could still be observed after 120 hours. IL-10 and MP obviously inhibited the expression of ICAM-1, which became more obvious when IL-10 and ICAM-1 were combined. Conclusion ICAM-1 plays a role in the early pathological stage of injury. It is one of the important factors that result in secondary spinal cord injury. An application of IL-10 and MP to block the expression of ICAM-1 in the early stage of SCI is conducive to the relieve of the damage.