摘要
①目的了解椎间盘中Ⅳ型胶原的表达及其与椎间盘退变的关系。②方法利用亲和细胞免疫组织化学技术及苏木精-伊红组织化学染色技术,观察Ⅳ型胶原在不同年龄组及退变手术椎间盘组织冷冻切片中的表达和变化情况,结合形态学变化,判断其与椎间盘退变的关系。③结果Ⅳ型胶原仅在青少年椎间盘中表达,主要分布在髓核细胞周围,且随年龄的增加,阳性染色细胞数明显增加;退变手术组及其他年龄组无Ⅳ型胶原表达;青少年组及成年组纤维环及软骨终板结构良好,而髓核存在明显的显微裂痕。④结论Ⅳ型胶原出现并存在于青少年椎间盘,是椎间盘髓核细胞对有害刺激的早期反应,可以作为椎间盘退变的早期指标之一。
Objective To investigate the expression of type-Ⅳ collagen and its relationship with degeneration of intervertebral disc. Methods The expressions of type-Ⅳ collagen in frozen sections of the discs collected from an operation for degenerative lesion and different age-group subjects were analyzed by using LAB immunohistochemical technology. Results Collagen type Ⅳ presented only in the discs of adolescents, which surrounded mainly the chondrocyte-like cells of the nucleus pulposus. The proportion of positively stained cells increased significantly with age. No positive reaction signals were found in age other groups. Conclusion Type-Ⅳ collagen occurs in the disc of adolescents is an early cellular reaction to harmful stimulation, which can be served as a biomarker of early degeneration of human lumbar intervertebral discs.
出处
《青岛大学医学院学报》
CAS
2005年第3期207-209,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅九五攻关课题(1997-26)
关键词
胶原Ⅳ型
椎间盘
免疫组织化学
collagen type Ⅳ
intervertebral disc
immunohistochemistry
