3种检测泌尿生殖道沙眼衣原体方法的比较

COMPARISON OF THREE DETECTION METHODS OF CHLAMYDIA TRACHOMATIS INFECTION IN UROGENITAL TRACT

①目的比较3种泌尿生殖道沙眼衣原体实验室检测方法的敏感度和特异度。②方法用细胞培养法、酶联免疫吸附测定(ELISA)法和聚合酶链反应(PCR)结合反向杂交技术,分别对201例非淋菌泌尿生殖道炎症病人的尿道(男性)或宫颈拭子(女性)以及血清标本进行检测,对3种方法的检测结果进行比较。③结果细胞培养法检测出阳性标本49例(24.38%),ELISA法检测出阳性标本38例(18.91%),PCR结合反向杂交技术检测出阳性标本51例(25.37%)。以细胞培养法检测结果为金标准,PCR结合反向杂交技术的敏感度和特异度分别为100.00%和98.68%;ELISA法则为73.47%和98.68%。PCR结合反向杂交技术和细胞培养法检测结果无显著性差异(χ2=0.50,P>0.05),两者与ELISA法检测结果比较均有显著性差异(2χ=8.47、6.67,P<0.05)。④结论PCR结合反向杂交技术具有较高的敏感度和特异度,可以用于临床检验。

Objective To compare the sensitivity and specificity of three methods in detecting chlamydia trachomatis infection in urogenital tract. Methods Specimens from male urinary tract （201 cases） and female cervix were collected and detected respectively by means of cell culturing, ELISA, PCR combining reverse cross blot hybridization. The results were compared. Results Forty nine positive specimens were found by conventional culture medium （24.38%）, 38 by ELISA （18.91%）, and 51 by PCR and reverse cross blot hybridization （25.37%）. PCR combining reverse cross blot hybridization had a sensitivity of 100.00% and a specificity of 98.68%, while ELISA had a sensitivity of 73.47% and a specificity of 98.68%. In comparison with these three laboratory diagnosis, the difference between the PCR combining reverse cross blot hybridization and cell culturing was not significant （x^2=0.50, P〉0.05）, while the difference between the PCR and reverse combining blot hybridization and ELISA was significant （x^2=8.47,6.67; P〈0.05）. Conclusion PCR combining reverse cross blot hybridization is a sensitive and quick method in the diagnosis of urethritis. Therefore, it is a useful technology in clinical examination.