菜豆下胚轴质膜微囊和液泡膜微囊体外热稳定性的研究

IN VITRO HEAT STABILITY OF PLASMA MEMBRANE VESICLES AND TONOPLAST MEMBRANE VESICLES ISOLATED FROM THE HYPOCOTYLE OF PHASEOLUS VULGARIS

从抗热潜势较高的“８５ＣＴ－４９７６２”菜豆（Ｐｈａｓｅｏｌｕｓ ｖｕｌｇａｒｉｓ Ｌ．）的下胚轴分离出微粒体、质膜微囊和液泡膜微囊。通过比较正常细胞的膜微囊、热锻炼细胞的膜微囊以及热锻炼过程中放线菌酮抑制了蛋白质合成的细胞膜微囊的质子泵在体外的热稳定性，发现热锻炼可使细胞膜微囊质子泵体外热稳定性提高，热锻炼过程中合成的蛋白质与此效应有关。进一步分析膜外周蛋白对质子泵体外热稳定性的影响，证明热激蛋白ＨＳＰ７０ 和小分子量热激蛋白（ＬＭＷ

Microsome,plasma membrane vesicles and tonoplast membrane vesicles were isolated from the hypocotyles of Phaseolus vulgaris L.85CT 49762,with very high heat tolerance optential.Comparing the H + pump heat stability in vitro of the vesicles from the heat acclimated cells and the cells in which protein synthesis was inhibited by actidion during heat acclimation with that of normal cells,the authors found that heat acclimation could increase the heat stability of membrane vesicles , and that the heat shock proteins synthesized during heat acclimation were related to the effect.The authors further analysed the role of membrane peripheral proteins on H + pump thermotolerance of membrane vesicles,and proved that heat shock protein HSP 70 and low molecular weight heat shock protein (LMW HSP) were able to protect H + pump from heat destruction.