授粉诱导蝴蝶兰雌蕊中乙烯合成和ACC氧化酶基因表达

POLLINATION INDUCED ETHYLENE SYNTHESIS AND 1 AMINOCYCLOPROPANE 1 CARBOXYLATE OXIDASE GENE EXPRESSION IN THE GYNOECIUM OF PHALAENOPSIS ORCHID FLOWER *

对蝴蝶兰（Ｐｈａｌａｅｎｏｐｓｉｓ “Ｇｅｎｅｒａｌｋｕ”ｈｏｒ．）在授粉后乙烯的合成和１－氨基环丙烷－１－羧酸（ＡＣＣ）氧化酶基因的表达进行了研究。实验结果显示在授粉后１２、２４ 和４８ ｈ，柱头和花柱中乙烯的产生和ＡＣＣ氧化酶ｍ ＲＮＡ 的积累显著下降，而子房中则明显上升，表明授粉后雌蕊中乙烯的产生与ＡＣＣ氧化酶基因的表达密切相关。此外，授粉后雌蕊的柱头中合成的乙烯相对量最多，花柱次之。

Pollination induced ethylene production plays an important role in regulating flower development.As gynoecium is one of the more important parts in Phalaenopsis orchid flower,the authors have investigated ethylene production and 1 aminocyclopropane 1 carboxylate (ACC) oxidase gene expression in gynoecium following pollination.Experimental studies revealed that ethylene production and ACC oxidase mRNA accumulation decreased steadily in stigma and style,in contrast,exhibited a crescendo increase in the ovary at 12,24 and 48 h after pollination,indicating a close relationship between ethylene production and ACC oxidase gene expression in the gynoecium.In addition ethylene production was found most in stigma and least in ovary among the three parts after pollination.