非放射性原位杂交技术检测胃癌组织c-erbB-2和c-myc癌基因扩增的研究

c-erbB-2 and c-myc expression is related with pathological progression of gastric cancer：results of a non- radioactive in sire hybridization

目的研究c—erbB—2和c—myc癌基因在胃癌组织中扩增的意义。方法应用非放射性原位杂交技术检测81例胃癌标本中c—erbB—2和c—myc的扩增情况。结果C—erbB—2和c—myc在胃癌中的扩增率分别为50．6％和67．9％。c—erbB—2基因与胃癌组织分化程度、浸润深度及淋巴结转移有关（P〈0．05），分化程度越差、浸润深度越深、淋巴结有转移，c—erbB—2基因表达率越高，而C—myc癌基因与胃癌组织分化程度、浸润深度及淋巴结转移无关。两基因的扩增具有显著的相关性（x^2=7．26，P〈0．01）。结论c—erbB—2和c—myc扩增是胃癌发生过程中的一个重要的生物学因素，且两者具有较好的协同作用。

AIM To study the significance of c - erbB - 2 and c - myc oncogene expression in gastric cancer. MEITHODS 81 gastric cancer specimens were studied for c - erbB - 2 and c - myc oncogene amplification using non - radioactive in situ hybridization method.RESULTS The amplification rates for c- erbB- 2 and c- myc were 50.6% and 67.9% ,respectively, and there were significant correlation in the amplification of these two genes（x^2 =7.26, P〈0.01）. CONCLUSIONS The amplification of c-erbB-2 and c - myc may play an important role in gastric cancer development, and these two genes may have synergistic effect.