摘要
构建了来自根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍ ｅｆａｃｉｅｎｓ） Ｔ－ＤＮＡ 的细胞分裂素基因（Ｔ－ｃｙｔ）启动子驱动下的ＧＵＳ基因的表达质粒，并用以转化烟草（Ｎｉｃｏｔｉａｎａ ｔａｂａｃｕｍ ｃｖ． Ｗ ３８）和马铃薯（Ｓｏｌａｎｕｍ ｔｕｂｅｒｏ－ｓｕｍ Ｌ． ｃｖ． Ｄｅｓｉｒｅｅ），研究其在转基因植物中表达的定位。结果表明，Ｔ－ｃｙｔ启动子在转基因植株的根、茎、叶、块茎和萌发的种子中均可表达。其中在茎和块茎中的表达是不均一的：在维管束部分表达较强，在侧芽或叶柄的生长点及块茎的芽生长点表达活性较高。此外，在培养基中加入０．１ ｍ ｇ／ＬＢＡＰ，转基因烟草茎中ＧＵＳ基因的表达活性增强，而对ＮＡＡ 没有明显的反应。
The location of GUS gene expression under control of T cyt gene (gene 4 of T DNA coding isopenteryl transferase) 5′ region in transgenic tobacco(Nicotiana tabacum cv. W38) and potato(Solanum tuberosum L. cv. Desiree) plants was examined with biochemical assays. The results showed differential distribution in various organs and different cell types. The highest levels of GUS activity were found in tobacco stem where axillary bud was initiated and potato buds on tubers. Moreover,the expression of T cyt promoter/GUS was found to be inducible in transgenic tobacco stem with cytokinin rather than auxin treatment. Additionally,the level of expression was high in the wounded leaf of transgenic potato. It was suggested that T cyt promoter may be selectively induced by some exogenous plant hormones.
基金
国家自然科学基金
