马尾松毛虫质型多角体病毒替代寄主筛选的研究

SCREENING OF DpCPV'S SUBSTITUTIONAL HOSTS

用ＤｐＣＰＶ感染８种鳞翅目昆虫的试验结果表明，棉铃虫最敏感，感染率达９７％。ＤｐＣＰＶ对棉铃虫的毒力ＬＣ５０为７．６５×１０４ＣＰＢ／ｍｌ，浓度与感染死亡率相关性及回归方程均达到显著性。用该虫生产的Ｈａ－ＤｐＣＰＶ的产量平均为８．１２５亿ＣＰＢ／条，与用原寄主马尾松毛虫生产的平均为７．９４亿ＣＰＢ／条基本相等。Ｈａ－ＤｐｏＰＶ对马尾松毛虫的毒力ＬＣ５０为１．５×１０４ＣＰＢ／ｍｌ，而ＤｐＣＰＶ对马尾松毛虫的毒力ＬＣ５０为３．８６×１０５ＣＰＢ／ｍｌ，两者相比，毒力没有下降。

species of tepidoptera insects were treated with DpCPV, the result showed that Heliothis armigera was the most susceptible one to DpCPV, the infected rate was up tO 97%, so it was screened for the best substitutional host of DpCPV. The median lethal concentration of DpCPV to Heliothis armigera was 7. 65 ×104CPB/ml, the correlativity between concentration and infected-lethal rate and their reperession equation were significant. The out put of Ha-DpCPV Produced from Heliothis armigera as that of DpCPV, the former was averaged 8. 125× 108CPB Per head, the later was averaged 7. 94 × 108CPB per head. The compeison between the toxicity of Ha-DpCPV and DpCPV to Dendrolimus showed that Ha-DpCPV's tokicity was not relieved, the median letal concentration of Ha-DpCPV was 1.5×104CPB/ml, and the one of DpCPV was 3. 86 × 105CPB/ml.