实时荧光定量RT-PCR检测良性前列腺增生与前列腺癌相关基因表达的临床意义

Study on the expression of BPH and prostate cancer related genes using real-tim e quantitative RT-PCR

目的应用实时荧光定量RT-PCR检测良性前列腺增生(BPH)与前列腺癌相关基因表达。方法通过实时荧光定量RT-PCR对9个前列腺癌相关基因(KLK3、KLK2、KLK11,pim-1,hepsin,PSMA,AR,p27,IGF-1)在前列腺正常组织、BPH组织和癌组织(PCa)定量表达,分析其特异性的差异。结果检测9个选定相关基因中发现KLK2,pim-1,AR和IGF-1在这3种组织中的表达差异有统计学意义(P<0.01)。结论KLK2,pim-1,AR和IGF-1作为前列腺癌组织特异性的分子标记物组合,有望提高前列腺癌早期诊断的准确性。

Objective To determine the expression of prostate hyperplasia- and cancer-related genes by real-time quantitative RT-PCR and to screen for those which can be used for clinical diagnosis of prostate cancer. Methods Using real-time quantitative RT-PCR, the expression of 9 genes including KLK3, KLK2, KLK11, pim-1, hepsin, PSMA, AR, p27, IGF-1 in 3 prostate tissues, ie, normal tissue, BPH tissue and prostate cancer tissue, were quantitated and their specifically differential expressions were analyzed. Results Of the 9 genes tested, 4 genes, ie, KLK2, pim-1, AR and IGF-1, were differentially expressed in the 3 tissues. The differences were statistically significant （P〈0.01）. Conclusions The combination of KLK2, pim-1, AR and IGF-1 which are specific molecular markers of prostate cancer tissue may improve the rate of early diagnosis of prostate cancer.