nm23H1、VEGF表达与前列腺癌转移和生存率的关系

The correlation of nm23H1,VEGF expression with tumor metastasis and survival rat e in prostate cancer

目的研究前列腺癌nm23H1和VEGF的表达,探讨其与前列腺癌发生、转移和生存率的关系。方法用免疫组化法检测41例前列腺癌切除标本中癌组织和10例癌周组织nm23H1和VEGF表达;用CD31标记血管内皮细胞,计数肿瘤组织的微血管密度(MVD)。结合临床资料分析nm23H1、VEGF与TNM分期、病理分级、有无骨转移和术后生存率的相关性。结果前列腺癌组织中nm23H1和VEGF阳性表达率分别为70.7%(29/41)和68.3%(28/41),癌周组织中分别为10.0%(1/10)和30.0%(3/10),差异均有统计学意义(P<0.01)。前列腺癌MVD平均为75.1±12.9/mm2,癌周组织MVD平均为32.1±8.5/mm2(P<0.01)。nm23H1阳性表达者骨转移率低,阴性表达者骨转移率高,两组间差异有统计学意义(P<0.01)。VEGF阳性表达者骨转移率高,阴性表达者无骨转移,两组间差异有统计学意义(P<0.01)。nm23H1高表达时生存率高,低表达时生存率低;VEGF高表达时生存率低,低表达时生存率高。结论nm23H1基因可抑制前列腺癌发生和转移,提高前列腺癌患者的生存率。VEGF高表达可促进前列腺癌的血管和淋巴管形成,导致癌细胞转移,使患者生存率降低。

Objective To study the expressions of non-metastatic gene 23H1 （nm23H1） and vascular endothelial growth factor （VEGF） in prostate cancer tissues and to explore their relationship with tumor development, tumor metastasis and patients＇ survival rate. Methods Using immunohistochemical method, 41 prostate cancer specimens and 10 normal tissue specimens （controls） were tested for expressions of nm23H1 and VEGF. Using CD31 for marking vascular endothelial cell, the tumor microvessel density （MVD） was calculated and the patients＇ survival rate after operation was investigated, The correlation of nm23H1, VEGF expression and MVD with TNM staging, pathologic grading and bone metastasis was analyzed in combination with the clinical data. Results The positive expression rates of nm23H1 and VEGF in prostate cancer were 70.7% （29/41） and 68.3% （28/41）, while they were 10.0% （1/10） and 30.0% （3/10） in controls, indicating significant difference between them （P〈0.01）. The mean MVD in prostate cancer tissue was 75.1±12.9/mm^2, while it was 32.1±8.5/mm^2 in the controls （P〈0.01）. With positive expression of nm23H1, the bone metastasis rate was low, while with negative expression of nm23H1, the bone metastasis rate was high. The difference was statistically significant （P〈0.01）. Patients with positive VEGF expression had higher bone metastasis rate, while those with negative VEGF expression had no bone metastasis. The difference was statistically significant （P〈0.01）. The higher the nm23H1 expression, the higher the survival rate. By contrast, the higher the VEGF expression, the lower the survival rate. Conclusions Gene nm23H1 can suppress prostate cancer development and metastasis, therefore, improve the survival rate. High VEGF expression in prostate cancer can promote the forming of blood and lymphatic vessels, causing tumor metastasis, which can reduce the survival rate.