氧化苦参碱对石英作用肺泡巨噬细胞保护效应的初步研究

Preliminary Study of Protective Effects of Oxymatrine on Quartz Treated Macrophage

目的探讨氧化苦参碱对石英作用肺泡巨噬细胞(AM)的保护效应.方法用支气管肺灌洗方法收集大鼠AM并培养,用小鼠胸腺细胞增殖法和双抗夹心法分别测定AM培养上清液中的白细胞介素-1(IL-1)、肿瘤坏死因子α(TNF-α)的含量;用2,4二硝基苯肼比色法测定AM培养上清液中乳酸脱氢酶(LDH)的含量;用TBA比色法测定AM脂质过氧化终产物丙二醛(MDA)的含量.结果氧化苦参碱为200～1 600 μg/ml时可以降低石英对AM的作用,AM TNF-α的表达水平随氧化苦参碱剂量的增加而从20.11 ng/ml下降到9.44 ng/ml,且差异有显著性.当浓度达到3 200 μg/ml时,药效略减弱;IL-1的表达水平也随氧化苦参碱剂量的增加而使其液闪计数cpm值从3 051.67降低到1 603.35,尤其是在800～1 600 μg/ml时有明显下降;对MDA和LDH也显示了随药物剂量的增加而趋于下降,但药效相对较弱.结论氧化苦参碱能抑制石英诱导AM分泌TNF-α和IL-1,同时还具有抗石英对巨噬细胞的氧化作用和保护巨噬细胞膜的功能.

Objective The protective effects of oxymatrine were evaluated for quartz on alveolar macrophage （AM）. Methods The cells were collected by washing with physiological saline from rat bronchia-alveolar and cultured. The amount of interleukin-1 （IL-1） and tumor necrosis factor-α （TNF-α） in culture medium was determined by the method of thymus cell proliferation and double resistant with filling. The amount of malonyl dialdehyde （MDA） and activity of lactic dehydrogenase （LDH） in the cell were also determined by the method of thiobarbituric acid assay and 2, 4-dinitropheny-hydrazin colorimetry. Results Oxymatrine from 200～1 600 μg/ml may lower the level of AM to secrete TNF-α from 20.11 to 9.44 with the dosage increase, but the effect was weakened when the drug dosage was 3 200 μg/ml. The IL-1 was also descended from 3 051.67 to 1 603.35 with the dosage increase, especially the effect has distinctively lowered when the drug dosage was 800～ 1 600 μg/ml. The effects of oxymatrine on MDA and LDH were also the same, but were relatively weakened. Conclusions The oxymatrine could restrain AM of quartz-derive to express TNF-α and IL-1, meanwhile might also resist the lipid peroxidation of AM of quartz-derive and protect the function of AM membrane.