肺鳞癌与人乳头瘤病毒相关性研究

The Studies on Human Papillomavirus Infection in Squamous Cancer of the Lung

目的:了解肺鳞癌组织中人乳头瘤病毒存在情况,并分析人乳头瘤病毒与肺癌发生部位、性别因素、角化程度、吸烟因素的关系。方法:采用地高辛标记的DNA探针对肺癌组织进行原位杂交检测,同时结合临床资料分析多种因素的相关性。结果:肺鳞癌组织,鳞状上皮生化组织、粘膜慢性炎组织HPV检出率分别为48.4%,27.4%,5.6%,各组比较差异显著(P<0.01),中心型肺癌与周围型肺癌组织HPV检出率分别为50.6%和4.4%,两组比较差异显著(P<0.01)。男女两组HPV检出率分别为46.1%和54.3%,差异无显著性(P>0.05)。吸烟组HPV检出率为50.6%,非吸烟组为43.9%,差异不显著(P>0.05)。结论:肺癌组织中存在一定比例HPV感染,HPV感染与肺鳞癌关系密切,中心型肺癌与HPV感染密切相关,HPV感染与性别及吸烟因素未发现明显相关性。人乳头瘤病毒感染可能与肺癌发生发展有关。

Objective： To investigate the relationship between human papillomavirus （HPV） infection and lung cancer, observe the distribution of HPV-DNA in the tissue of lung cancer and to analyze the interrelationship among human papillomavirus, smoke, sex and the histological stage of lung cancer. Methods： Tissue of lung cancer was obtained by surgical resection or biopsy. The tissue specimens were detected by in situ hybridization techniques for human papillomavirus types 6B/11, 16, 18 and 31 using digoxingemin labelled DNA probe. The studies on the mechanisms involved in pulmonary carcinogenesis associated with HPV infection. Results： a, The positive sign was mainly located in the nuclei of the cells. The rate of 48.4% of squamous cell carcinomaoma, 27.4% of metaplasia and 5.6% of chronic inflammation tissue were positive for HPV-DNA by in situ hybridization （P〈0.01）; b, The positive rate of HPV in the central type group and peripheral typecontrol group was 50.6% and 4.4%, respectively （P〈0.01）. c. No significant difference in positive rate of HPV in smoke, sex and the histological stage of lung cancer were found in squamous cell carcinoma. Conclusion： The HPV-DNA is closely associated with squamous cell carcinoma of the lung, especially in the central type lung cancer. The correlationship between the HPV-DNA and smoke, the sex and the histological stage of lung cancer were not found.