哮喘儿童血清Clara细胞分泌蛋白、白介素-13的检测及意义

To detect and analyze Clara cell secretory protein and interleukin-13 in asthmatic children

目的:探讨哮喘儿童血清Clara细胞分泌蛋白(CCSP或CC10或CC16)、白介素-13(IL-13)的变化和临床意义。方法;采用酶联免疫吸附法检测22例哮喘急性发作期儿童血清CCSP、IL-13水平,经治疗后于缓解期采血复查,并与20例健康儿童作对照观察。结果:哮喘急性发作期、缓解期儿童血清CCSP水平均较健康对照组儿童低(P<0.01),在急性发作期尤为明显。哮喘急性发作期儿童血清IL-13明显高于对照组(P<0.001),缓解期降至正常。无论是急性发作期还是缓解期血清CCSP与IL-13无相关性(P>0.05)。结论:CCSP具有抗炎作用,CCSP的减少可诱导或加重哮喘儿童的呼吸道炎症。IL-13参与哮喘的发病过程,在呼吸道炎症的形成、呼吸道重塑中起重要作用。

Objective ： To approach the clinical significances of Clara cell secretory protein （CCSP or CC10 or CC16） and interleukin-13 （IL-13） in bronchial asthma children. Methods：Serum were collected from 22 cases during asthmatic attacks and in stable conditions, and 20 health children. Both serum CCSP and IL-13 concentrations were measured by enzyme-linked immunosorbent assay （ELISA）, Results ：It was found that asthmatic children had significantly lower levels of CCSP in serum during asthmatic attacks （P〈0. 001） or in stable conditions （P〈0. 01） as compared to healthy ones, especially those in acute asthma, Serum IL-13 levels in asthmatic attacks were significant higher than that in stable conditions（P〈0. 001）. The concentrations of IL-13 showed no significantly difference between the patients in stable conditions and health children（P〉0. 05）, Whether in acute asthma or in stable conditions ,the serum CCSP and IL- l3 levels of patients no correlation . Conclusion：CCSP may play a role in controlling inflammation in the airway, the decrease of CCSP may give an accelerating cause for further aggravation of inflammatory response in chronic asthma. IL-13 may participate in pathogenesis of asthma, and they may play an important role in the airway inflammation.