高通量透析膜蛋白质通透性的研究

Study on the protein permeability in high-flux dialysis membranes

目的评估高通量透析膜的蛋白质通透性。方法所有的透析模式均设为单纯超滤,在应用不同的透析膜透析时于透析器的出液口留取滤出液标本,同时进行蛋白质浓度测定和十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SDS-PAGE)作蛋白质电泳分析。SDS-PAGE采用银染色的方法并根据Melzer的方法作了改良,结果应用激光吸光度测定仪进行条带分析。结果透析膜滤出液中蛋白质条带与经肾小球膜滤出的蛋白质相似,含IgG、转铁蛋白、视黄醇结合蛋白和β2-微球蛋白。由于不同的透析膜的分子截留量、电荷和吸附能力不同,其蛋白质条带的分布亦不同。随着透析的进行,所有膜的通透性都逐渐下降。结论高通量透析膜的蛋白质通透性与肾小球滤过膜相似,但受孔径、表面所带电荷、吸附能力和透析时间的影响而发生改变。与人体的肾小球滤过膜不同,随着透析的进行,透析膜的蛋白质通透性下降。

Objective To evaluate the protein permeability of different high-flux dialysis membranes. Methods The filtrate was sampled from the dialysate compartment of different dialysers during the routine dialysis therapy. SDS-PAGE analysis was performed with silver staining method according to the modification of Melzer and consecutive laser densitometry. Before sampling all the dialysis mode was changed to pure ultrafiltration. Results The protein pattern of filtrate from dialysis membranes was similar to that of the glomerular membrane containing IgG, transferrin, albumin, retinol binding protein and beta-2-microglobulin. Comparing different membranes there were considerable differences depending on cut-off, charge and adsorption capacity of the particular membrane. In all membranes, permeability of proteins decreased during one treatment session. Conclusion Protein permeability of high-flux dialysis membranes is similar to the glomerular membrane but modified according to poresize, surface charge, adsorption and time on dialysis. In contrast to the glomerular membrane, in each of the investigated membranes protein permeability decreases during the session.