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大鼠胚胎发育后期胰腺中Mesothelin的表达 被引量：5

Expression of Mesothelin during later rat pancreatic development
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摘要 目的:探讨大鼠胰腺胚胎发育后期胰岛形成及β细胞功能完善相关的基因表达调控.方法:采用高密度寡核苷酸芯片(A ffym etrix芯片)对孕15.5(E15.5)和E18.5胰腺进行基因转录水平分析并用RT-PCR验证基因在大鼠胰腺E12.5,E15.5,E18.5,初生和成年这五个不同发育时期的表达情况.结果:在差异或特异表达的基因中与胰腺细胞分化相关的转录因子和信号分子表达均下调,而与胰岛结构形成及β细胞代谢功能完善相关的基因表达上调,其中细胞黏附分子Mesothelin是在E18.5特异表达的基因,RT-PCR亦显示Mesothelin在大鼠胰腺胚胎发育后期特异性高表达.结论:Mesothelin可能对胰岛结构的形成和功能的完善及维持有重要作用. AIM： To investigate the molecular mechanism involved in the islet formation and maturation of metabolic regulation by β cells. METHODS： The expression patterns of pancreas at embryonic day 1.5.5 （E15.5） and E18.5 were compared using the GeneChip Rat Expression Set 230 A. Based on the expression profiles of genes, the expression of mesothelin at different stages of rat pancreatic development was detected by RT-PCR. RESULTS. Out of 1319 genes of different expressions, the transcription factors and signal components related to cell differentiation were downregulated at the mRNA level while the genes related to islet formation and the function of metabolic regulation were upregulated. Mesothelin was specifically expressed at E18.5 pancreas, which was confirmed by RT-PCR. CONCLUSION： It is from El5.5 to El8.5 that the islet is gradually formed and undergoes further remodeling and maturation. Mesothelin may be a novel regulator of the islet formation and maturation.
作者 滕丽萍 胡静静 袁庆新 袁栎 周锦勇 郭静 程梅 刘超 德伟
机构地区 南京医科大学生物化学与分子生物学系 南京医科大学第一医院内分泌科
出处 《第四军医大学学报》 北大核心 2005年第17期1570-1572,共3页 Journal of the Fourth Military Medical University
基金 江苏省科委应用基础项目(BK2001119)
关键词 MESOTHELIN 胰岛 RT—PCR 高密度寡核苷酸芯片 mesothelin islet RT-PCR GeneChip
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献10

  • 1Davidson EH, McClay DR, Hood L. Regulatory gene networks and the properties of the developmental process[J]. PNAS, 2003;100: 1475-1480.
  • 2Gu GQ, Wells JM, Dombkowski D, et al. Global expression analysis of gene regulatory pathways during endocrine pancreatic development[J]. Development, 2004; 131: 165-179.
  • 3Perez SE, Cano DA, Dao-Pick T, et al. Matrix metalloproteinases 2 and 9 are dispensable for pancreatic islet formation and function in vivo[J]. Diabetes, 2005;54(3):694-701.
  • 4倪雪峰,袁栎,程志祥,熊俊,顾冬民,朱自路,周锦勇,仲燕,德伟.巢蛋白在大鼠胚胎、新生及成年胰腺中的表达变化[J].第四军医大学学报,2004,25(3):193-196. 被引量：21
  • 5Wilding L, Gannon M. The role of pdx1 and HNF6 in proliferation and differentiation of endocrine precursors[J]. Diabetes Metab Res, 2004; 20: 114-123.
  • 6Odom DT, Zizlsperger N, Gordon DB, et al. Control of pancreas and liver gene expression by HNF transcription factors[J]. Science, 2004; 303(5662): 1378-1381.
  • 7Ashizawa S, Brunicardi FC, Wang XP. PDX-1 and the pancreas[J]. Pancreas, 2004;28(2):109-120.
  • 8Brink C. Promoter elements in endocrine pancreas development and hormone regulation[J]. Cell Mol Life Sci, 2003; 60(6):1033-1048.
  • 9Kandeel F, Smith CV, Todorov I. Advances in islet cell biology[J]. Pancreas, 2003;27(3):64-78.
  • 10Rump A, Morikawa Y. Binding of ovarian cancer antigen CA125/MUC16 to mesothelin mediates cell adhesion[J]. J Biol Chem, 2004;279(10):9190-9198.

二级参考文献5

  • 1[1]Herrmann H, Aebi U. Intermediate filaments and their associates: Multi-talented structural elements specifying cytoarchitecture and cytodynamics[J]. Curr Opin Cell Biol, 2000;12:79-90.
  • 2[2]Zulewski H, Abraham EJ, Gerlach MJ, et al. Multipotential Nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes [J]. Diabetes, 2001;50: 521-533.
  • 3[3]Huang H, Tang X. Phenotypic determination and characterization of nestin-positive precursors derived from human fetal pancreas[J]. Lab Invest, 2003;83(4):539-547.
  • 4[4]Lendahl U, Zimmerman LB, McKay RD. CNS stem cells express a new class of intermediate filament protein[J]. Cell, 1990; 60:585-595.
  • 5[6]Lumelsky N, Blondel O, Laeng P, et al. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets [J]. Science, 2001;292: 1389-1394.

共引文献20

同被引文献31

  • 1张鑫,彭品贤,吕嘉春,梁建辉,吴建军.Paxillin基因在肺癌组织中的表达研究[J].广州医学院学报,2004,32(2):4-7. 被引量：10
  • 2刘长锁,陈乃宏.桩蛋白的结构与功能[J].生命的化学,2005,25(3):208-210. 被引量：12
  • 3吴小华,王小玲,张立芳,李利,王永军,郭燕红,郭清,杨波,黎海莉.卵巢上皮性肿瘤组织中间皮素和CA_(125)表达及其相关性研究[J].中华妇产科杂志,2005,40(10):709-711. 被引量：16
  • 4宋和存,郭毅,李慧.低雌激素水平在卵细胞和胚胎发育中的作用[J].中国现代医学杂志,2006,16(3):359-361. 被引量：5
  • 5Gubbels JA, Belisle J, Onda M, et al. Mesothelin-MUC16 binding is a high affinity, N-glycan dependent interaction that facilitates peritoneal metastasis of ovarian tumors[J]. Mol Cancer, 2006,5 (1): 50.
  • 6Bergan L, Gross JA, Nevin B, et al. Development and in vitro validation of anti-mesothelin biobodies that prevent CA125/Mesothelin-dependent cell attachment[J]. Cancer Lett, 2007,255:263-274.
  • 7Scholler N, Garvik B, Hayden-Ledbetter M, et al. Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery[J]. Cancer Lett, 2007,247:130-136.
  • 8Habener JF, Kemp DM, Thomas MK. Transcriptional regulation in pancreatic development [J]. Endocrinology, 2005,146(3): 1025-1034.
  • 9Kim SK,Hebrok M.Intercelluar signals regulating pancreas development and function [J].Genes Dev, 2001,15:111-127.
  • 10Mathis D,Vence L,Beniost C.Beta-Cell death during progression to diabetes [J].Nature,2001,13(6865):792-798.

引证文献5

二级引证文献3

第四军医大学学报
2005年 第17期

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