成人睾丸支持细胞的分离培养及其免疫豁免机制

The isolation and culture of Sertoli cells from adult testis and their immune privilege mechanism

目的:建立成人睾丸支持细胞的体外培养方法并探讨其免疫豁免机制。方法:依次用胰蛋白酶、胶原酶、透明质酸酶及脱氧核糖核酸酶消化制备成人睾丸支持细胞,以SABC染色法检测支持细胞上Fas-L和TGF-β1的表达。将其与成人脾细胞共同培养,用MTT比色法检测其对脾细胞增殖的抑制作用。结果:成人睾丸支持细胞占培养细胞总数的80%以上,活性可达90%。睾丸支持细胞上可表达Fas-L和TGF-β1,体外可抑制共培养的脾细胞增殖。结论:建立了培养成人睾丸支持细胞的方法,其免疫豁免机制可能与Fas-L和TGF-β1的表达有关。

AIM： To set up a method of isolation and culture of Sertoli cells from adult testis and to investigate their immune privilege mechanism. METHODS： Adult testis Sertoli cells were prepared successfully by digestion with trypsin, collagenase, hyaluronidase and DNase, and then the expression of Fas-L and TGF-β1 was examined by SABC staining. The Sertoli cells were cocultured with adult splenocytes to detect the inhibitory effect of Sertoli cells on splenocyte proliferation by MTT colorimetry, RESULTS： In cultured cells, Sertoli cells accounted for more than 80% of total cells. The activity of Sertoli cells reached 90%. Sertoli cells expressed Fas-L and TGF-β1 and could inhibit the proliferation of cocultured splenocytes in vitro. CONCLUSION： A method of isolation and culture of Sertoli cells from adult testis has been established. The immune privilege mechanism of Sertoli cells may be related to the expression of Fas-L and TGF-β1.