膀胱癌细胞CAR的表达对E1B缺失腺病毒抗瘤活性的影响

Effect of CAR Expression on Antitumoral Activity of E1B-Deleted Adenovirus in Bladder Cancer Cells

【目的】观察不同组织类型膀胱癌细胞株表达柯萨奇病毒腺病毒受体(coxsackieandadenovirusreceptor,CAR)与E1B缺失腺病毒(H101)体外感染力及体内抗瘤活性的关系。【方法】体外通过流式细胞仪法(FCM)检测不同组织类型膀胱癌细胞株表面CAR表达,用MTT法测定病毒对它们的抑制率,以观察CAR表达与H101感染力的关系;利用裸鼠移植瘤模型,观察H101对不同移植瘤的抗瘤活性。【结果】膀胱癌细胞上CAR的表达量与H101的感染力呈正相关(r=0.952);动物移植瘤实验结果显示,H101对CAR表达高的SCaBER移植瘤的抑制率较高,有效持续的时间也较长。【结论】膀胱癌细胞株上不同的CAR表达可直接影响H101的体内外抗瘤活性。

[Objective] To explore the relationship between the expression of Coxsackie and adenovirus receptor （CAR） in bladder cancer cells and the infectivity in vitro, as well as the antitumoral activity in vivo of E1B-deleted adenovirus （H101）. [Methods]The amount of CAR in the different histological type of bladder cancer cells were detected by flow cytometry （FCM）, and inhibitory rate of H101 on bladder cells were determined by MTT assay. The relationship between CAR expression and the infectivity of H101 was explored. Human bladder transitional cell carcinoma cell line T-24 and human bladder squamous carcinoma SCaBER were used to establish a model of transplanted tumors, after injection of H101, the growth of the transplanted tumor was observed. [Results]The amount of CAR in different bladder cancer cells was different and positively related to viral infectivity （r = 0.952）. Meanwhile, the amount of CAR affects the antitumoral activity of H101 in vivo, not only in inhibitory rate, but also in time to progression. [Conclusion]The study showed the efficacy of H101 therapy were significantly affected by the expression of CAR in bladder cancer cells.