肺炎衣原体感染和高脂血症对心肌细胞NF-kappa B和AP-1的影响

Effects of Chlamydia pneumoniae infection and hyperlipidemia on the expression of NF-κB and AP-1 in myocardium

目的:探讨肺炎衣原体感染和高脂血症对心肌细胞炎症的影响。方法:用间接免疫荧光的方法检测肺炎衣原体感染或给予高脂饮食的C57BL/6J小鼠,观察NF-κB亚单位P50和c-Fos在小鼠心肌细胞中表达程度。结果:肺炎衣原体感染和高脂血症能引起心肌细胞中P50和c-Fos的激活。对照组心肌细胞核中未见P50和c-Fos的表达,而3个实验组心肌细胞核中都有不同程度的P50和c-Fos表达。实验组和对照组比较P<0.01,在3个实验组间无显著差异。结论:在肺炎衣原体感染和高脂血症形成的早期,心肌细胞的炎症通路已被激活。

AIM： To investigate the effects of Chlamydia pneumoniae infection and hyperlipidemia on the expression of NF-κB and AP- 1 in the myocardium. METHODS： The indirect immunofluorescence method was used to examine wild C57BL/ 6J mice infected with Cblamydia pneumoniae and fed with an atherogenic diet. The expression of the subunit of NF - κB, P50, and c - Fos in the murine myocardium was observed. RESULTS ： Chlamydia pneumoniae infection and hyperlipidemia induced the activation of NF- κB and AP- 1 in murine myocardium. P50 and c - Fos were not detected in the controls, but there were different levels of positive expression in the experiments （ P 〈 0.01, compared to the controls）. No statistical significance among the experi- ments was observed. CONCLUSION： In early time of Chlamydia pneumoniae infection and hyperlipidemia, the inflammatory pathway is already activated in the myocardium.