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气道给rhSOD对胎粪诱导肺损伤中NF-κB及MIP-1α表达的影响 被引量:2

Effect of rhSOD on pulmonary nuclear factor-kappa B and MIP-1α expression in meconium-induced acute lung injury
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摘要 目的:观察早期经气道给予重组人超氧化物歧化酶(rhSOD)对胎粪诱导大鼠肺NF-κB和炎症因子MIP-1α表达的影响,以探讨其在胎粪诱导肺损伤中的作用及其机制。方法:24只雄性SD大鼠,随机分为:(1)对照组(control),经气管插管注入生理盐水1mL/kg;(2)胎粪+生理盐水处理组(Mec/saline);(3)胎粪+rhSOD治疗组(Mec/rhSOD)。后两者先由气管插管注入20%新生儿胎粪生理盐水混悬液1mL/kg建立急性肺损伤模型,再分别经气管插管注入生理盐水1mL/kg或rhSOD20g.L-1.kg-1。24h后取材,RT-PCR法测定肺组织MIP-1αmRNA、Westernblotting法测定NF-κB蛋白表达改变,同时行支气管肺泡灌洗液(BAL)细胞计数。结果:Mec/saline组大鼠BAL细胞计数、肺组织MIP-1αmRNA和NF-κB蛋白表达均明显高于control组[(4.68±1.40)×109cells/Lvs(0.53±0.19)×109cells/L,3.60±0.75vs1.56±0.33,0.72±0.31vs0.23±0.21],(均P<0.01);Mec/rhSOD组大鼠BAL细胞计数、肺组织MIP-1αmRNA和NF-κB蛋白表达分别为(3.13±0.77)×109cells/L、2.20±0.39和0.44±0.21,均显著低于Mec/saline组(均P<0.01),但仍显著高于control组(均P<0.01)。结论:早期经气道给rhSOD可能通过抑制肺MIP-1α和NF-κB表达而减轻胎粪诱导的肺炎症反应。 AIM: To evaluate the role and mechanisms of recombinant human superoxide dismutase (rhSOD) in meconium- induced acute lung injury. (ALI) by evaluating pulmonary MIP- 1α and NF - κB expression. METHODS: 24 health male Sprage - Dawley rats were randomized to 3 groups (8, each group), followed by intratracheal (IT) administration with (1) saline at 1 mL/kg (control group); (2) 20% human newborn meconium suspension at 1 mL/kg, followed by aline at 1 mL/kg (Mec/ saline group) ; (3) 20% human newborn meconium suspension at lmL/kg, followed by rhSOD at 20 mg/kg (Mec/rhSOD group). The animal was killed 24 h after treatment. The measurements included the bronchoalveolar lavage (BAL) cell count, RT- PCR analysis of pulmonary MIP-1α mRNA expression, Western blotting analysis of pulmonary NF- κB expression, RESULTS: Meconium - induced ALI was characterized by increased BAL cell count, increased expressions of pulmonary MIP - 1α mRNA and NF - κB protein [ (4.68 ± 1.40) × 10^9 cells/L vs (0.53 ± 0. 19) × 10^9 cells/L, 3.60 ± 0.75 vs 1.56 ± 0.33, 0.72 ± 0.31 vs 0.23 ± 0.12, respectively in control rats, all P 〈 0.01 ]. IT administration of rhSOD early in the ALl rat significantly decreased meconium - indueed BAL cell count [ (3.13 ± 0.77) × 10^9 cells/L vs (4.68 ± 1.40) × 10^9 cells/L in Mec/saline rats, P 〈 0.01 ], inhibited the expression of pulmonary, MIP- 1α mRNA (2,20 ± 0.39 vs 3,60 ± 0.75, in Mec/saline rats, P 〈 0.01 ) and NF- κB protein (0.44 ± 0.21 vs 0.72 ± 0.31 in Mee/saline rats, P 〈 0.05). CONCLUSION: The early IT administration of rhSOD in ALl rat following meeoniunl aspiration protects lung from inflanunatory injury through inhibiting meconium - induced pulmonary MIP - let mRNA anti NF- κB protein expression.
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2005年第9期1724-1727,共4页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.30371498) 浙江省教育厅资助项目(No.20030304) 浙江省科技厅资助项目(No.2004C33019)
关键词 超氧化物岐化酶 胎粪 肺损伤 NF-ΚB 巨噬细胞炎性蛋白质1 Superoxide dismutase Meconium Lung injury NF- kappa B Macrophage inflammatory protein- 1
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参考文献11

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共引文献2

同被引文献14

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