磷酸二酯酶抑制剂IBMX对海马区cGMP合成的影响

Effect of phosphodiesterase inhibitor IBMX on cGMP production in gerbil hippocampus

目的:探讨伊菠丁基甲基黄嘌呤对海马区环磷酸鸟苷(cGMP)合成的影响。方法:嵌夹沙土鼠的双侧颈总动脉复制的脑缺血模型,应用免疫荧光组织化学染色方法。结果:脑缺血再灌注后,海马区cGMP合成增加。使用磷酸二酯酶(PDE)抑制剂IBMX,cGMP合成增加,cGMP阳性细胞主要分布于海马本部放射层及腔隙分子层。cGMP阳性细胞的数量与IBMX的剂量有关。结论:伊菠丁基甲基黄嘌呤增加cGMP的合成。

AIM： To observe the effects of IBMX on cGMP production in gerbil hippocampus after recircnlation following ischemia. METHODS： Bilateral occlusion of common carotid arteries and inununofluorescent staining methods in gerbil hippocampal tissue slice were used. RESULTS： Recircnlation following ischemia caused a rise in hippocampus cGMP concentration. IBMX increased cGMP production, cGMP positive cells mainly distributed in rediatum layer and molecular layer in the CAI subfield. CONCLUSION： IBMX increased cGMP production in gerbil hippocampus after recircnlation following ischemia.