刺五加干预后新生大鼠缺血缺氧脑组织前列腺素的代谢改变

Alteration of prostaglandin metabolism in brain tissue of newborn rats with hypoxic-ischemic brain damage after the intervention of manyprickle acanthopanax root

目的:探讨益气活血中药刺五加对新生大鼠缺血缺氧脑组织前列腺素代谢改变的影响。方法:实验于2003-04/08在延边大学医院儿科疾病研究室进行。取新生7d的Wistar大鼠36只,随机分成3组:①正常组(n=10):不干预。②缺氧缺血脑病组(n=13):麻醉后结扎左侧颈总动脉,1h后置于37℃密封缺氧箱内,以2.0～3.0L/min速度吸入含体积分数为0.08的氧气的气体,持续2h,制备脑组织缺血缺氧动物模型。③刺五加组(n=13):制备模型同前,模型成功后即刻腹腔注射刺五加(10mL/kg,5g/L)。3组动物在48h后取材,用放射免疫分析法测量新生大鼠脑组织中前列腺素血栓素B2,6-酮-前列腺素F1含量变化。结果:实验中6只动物死亡,30只进入结果分析。①脑组织6-酮-前列腺素F1含量:缺氧缺血脑病组显著高于正常组和刺五加组犤(22.23±5.95),(13.63±2.50),(10.62±1.40)ng/g,F=11.618,P<0.01犦,正常组和刺五加组无差异(P>0.05)。②脑组织血栓素B2含量:缺氧缺血脑病组显著高于正常组和刺五加组犤(13.67±2.35),(9.11±0.96),(10.58±1.80)ng/g,F=13.248,P<0.01犦,正常组和刺五加组无差异(P>0.05)。结论:缺血缺氧模型脑组织中血栓素B2和6-酮-前列腺素F1含量明显增高,说明存在血栓素及前列腺素代谢紊乱,易形成血栓。刺五加能显著降低脑组织中此两种因子的含量,对缺血缺氧脑组织前列腺素代谢方面有保护作用。

METHODS： The experiment was conducted in Laboratory ot Pediatrics Disease of Yanbian University Hospital from April to August 2003. Thirtysix new born Wistar rat aged 7 days old were selected, and divided randomly into 3 groups.①Normal group （n=10）： Without intervention.② Hypoxia ischemia group （n=13）： After anesthesia, ligation of left side carotid was done in rats, one hour later they were laid enclose hypoxia box with 37℃ at the speed of 2.0-3.0 L per minute absorbing oxygen at 0.08 volum number for 2 hours to make animal model with ischemia hypoxia in brain tissue. ③Manyprickle acanthopanax root group （n=13）： The model was the same as above. After the success of making model, manyprickle acanhopanax root injection was treated immediately. We took the materials from 3 groups after 48 hours. Then we tested the changes of thromboxane B2 （TXB2）, 6-K-PGF1 of prostaglandin metabolites in the brain. RESULTS： Thirty rats were involved in the results analysis, for 6 rats die during experiments.① The content of 6-K-PGF1 TXB2 in the brain tissue： It was significantly higher in hypoxia ischemia group than that in normal group and manyprickle acanthopanax root group [（22.23±5.95 ）, （13.63±2.50）,（10.62±1.40） ng/g, （F=11.618, P〈0.01）], and there was no difference between normal group and manyprickle acanthopanax root group （P 〉 0.05）. ② The content of TXB2： It was significantly higher in hypoxia ischemia group than that in normal group and manyprickle acantbopanax root group [（13.67±2.35）, （9.11±0.96）, （10.58±1.80） ng/g, （F=13.248, P〈 0.01）], and there was no difference between normal group and manyprickle acanthopanax root group （P 〉 0.05 ）. CONCLUSION： The content of TXB2 and 6-K-PGF1 significantly increases in model brain tissue with ischemia and hypoxia, which indicates that the metabolism disorder of throboxane and prostaglandin leads easily to the formation of thromhus. Manyprickle acanthopanax root can decrease siguific antly the content of those two factors in brain tissue, and have protective effect on prostaglandin metabolism of brain tissue with ischcmia and hypoxia.