多梗死性痴呆患者血清黏附分子与自由基代谢的相关性(英文)

Association between serum adhesion molecules and free radical metabolism in patients with multi-infarct dementia

背景:黏附分子与炎症反应密切相关,脑缺血后白细胞浸润引起的炎症反应及自由基损伤作用被认为是多梗死性痴呆发病的重要因素。目的:测定多梗死性痴呆患者血清黏附分子水平与氧自由基浓度,探讨两者之间的相关性。设计:病例-对照实验。单位:吉林大学第二医院神经内科。对象:选择2000-01/2004-10吉林大学第二医院神经内科住院多梗死性痴呆患者82例,其中轻度痴呆32例,中度痴呆21例,重度痴呆29例。正常对照组为同期同一医院自愿参加实验的体检人员,共23例。方法:采用酶联免疫法和电子自旋共振技术测定正常对照组、多梗死性痴呆患者血清可溶性胞间黏附分子、可溶性血管细胞黏附分子水平和氧自由基浓度,并分析病情严重程度对黏附分子水平及氧自由基浓度的影响。主要观察指标:①两组对象血清细胞间黏附分了-1含量。②两组对象血管细胞黏附分子-1含量。③两组对象血氧自由基浓度。结果:参加试验多梗死性痴呆患者82例和正常对照组23例,全部进入结果分析。①血清可溶性黏附分子、血管细胞黏附分子-1水平、氧自由基浓度:多梗死性痴呆组高于对照组犤(469.00±76.33),(196.00±45.91)μg/L;(1103.30±98.96),(601.00±76.30)μg/L;(4.018±1.656),(1.295±0.718)g/g,(t=5.517～6.754,P<0.01)犦。②血清细胞间黏附分了-1、血管细胞黏附分子-1含量及血自由基浓度与痴呆程度呈正相关(r=0.6594,r=0.6972,r=0.6494,P<0.05)血清细胞间黏附分了-1、血管细胞黏附分子-1含量与自由基浓度呈正相关(r=0.7147,r=0.7324,P<0.01)。结论:血清细胞间黏附分了-1、血管细胞黏附分子-1、氧自由基参与了多梗死痴呆病理生理发展过程,痴呆越重黏附分子水平、氧自由基浓度越高。

BACKGROUND： Adhesion molecules are closely associated with inflammation, Inflammation due to white blood cell （WBC） infiltration and free radical injury following brain ischemia are believed to he important factors contributing to the pathogenesis of multi-infarct dementia. OBJECTIVE： To determine the level of serum adhesion molecules and free radicals in patients with multi-infarct dementia to explore the relationship between their levels and multi-infarct dementia. DESIGN： A case-control trial. SETTING： Department of Neurology. Second Affiliated Hospital of Jilin University. PARTICIPANTS： Totally 82 patients with multi-infarct dementia were hospitalized in the Department of Neurology, Second Affiliated Hospital of Jilin University between January. 2000 and December 2004. These patients included 32 cases of mild dementia, 21 of moderate dementia, and 29 of severer dementia. The normal controls were 23 concomitant healthy volunteers who came for routine physical examination.METHODS： Enzyme-linked immunosorbent assay （ELISA） and electron spin resonance were used to determine the level of serum soluble intercellular adhesion molecules （ICAM） and vascular cell adhesion molecules （VCAM）, as well as oxygen free radical concentration in the normal controis and patients with multi-infarct dementia, and the association between the severity of the illness and the levels of adhesion molecules and oxygen free radicals was analyzed. MAIN OUTCOME MEASURES： Serum levels of 1CAM-1 and VCAM-1 and oxygen free radical concentration in the two groups. RESULTS： Totally 82 patients with multi-infarct dementia and 23 healthy controls were included in this study and all enter the result analysis. In multi-infarct dementia patients, the serum levels of soluble ICAM-1 and VCAM-1 and oxygen free radical concentration [（469.00±76.33）, （196.00±45.91） and （1 103.30±95.96）μg/L, respectively] were significantly higher than those of the control group [（601.00±76.30）, （4.018±1.656）, and （1.295±0.715）μg/g, respectively, t=5.517-6.75d, P 〈 0.01], and the 3 indices were positively correlated with the severity of dementia （r=0.659 d, r=0.697 2, r=0.649 d, respectively, P 〈 0.05）; serum ICAM-1 and VCAM-1 levels were positively correlated with the concentration of oxygen free radicals （r=-0.71d 7, r=0.732 d, respectively, P 〈 0.01）. CONCLUSION： Serum ICAM-1, VCAM-1 and oxygen free radicals might be implicated in the pathophysiological development of multi-infarct dementia, and their levels increase in parallel with the severity of dementia.