萎缩性胃炎及胃癌中垂体肿瘤转化基因和c-myc蛋白的表达及其二者的相关性

Expression and correlation between PTTG and c-myc protein in gastric carcinoma and chronic atrophic gastritis

目的研究垂体肿瘤转化基因(PTTG)基因和c-myc蛋白在胃癌发生发展中的作用及二者的相关性。方法采用免疫组织化学SP法检测90例正常胃组织、慢性萎缩性胃炎及胃癌组织中PTTG基因和c-myc蛋白的表达。结果PTTG基因和c-myc蛋白在正常胃组织中表达均为阴性,在慢性萎缩性胃炎、胃癌组织中表达明显高于正常胃组织(P<0.05),二者的表达强度在慢性萎缩性胃炎组与胃癌组间比较均有显著性差异(P<0.05)。二者的表达均与胃癌的病理组织学分级、临床分期及淋巴结转移有关(P<0.05),而与组织学类型无关(P>0.05)。PTTG基因和c-myc蛋白表达强度呈显著正相关(P<0.05)。结论PTTG基因和c-myc蛋白在胃癌的发生发展中有协同作用,可作为反映胃癌生物学行为的指标。

Objective To study the functions of PTTG gene and c-myc protein in the development of gastric carcinoma and the action between PTTG gene and c-myc protein. Methods Immunohistochemical staining （SP） was used to examine the expression of PTTG gene and c-myc protein in 90 cases of gastric specimens. Results Both PTTG gene and c-myc protein were negative in normal gastric mueosa. An increased expression of them were found in chronic atrophic gastritis and gastric carcinoma compared with normal gastric mucosa（ P 〈 0.05 ）. The expressive intensity of PTTG gene and c-myc protein were significant between chronic atrophic gastritis and gastric carcinoma（ P 〈 0.05 ）. The expressions of them were related to pathohistological grade,clinical stage and lymph node metastasis（P 〈 0.05 ）,but to histological type of gastric carcinoma（ P 〉 0.05 ）. There was a positive correlation between the expressive intensity of PTTG gene and c-myc protein（ P 〈 0.05 ）. Conclusion PTTG gene and c-myc protein play a cooperative role in the development of gastric carcinoma and may serve as useful markers for assessment of the biological behavior of gastric carcinoma.